EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.18.1.2 | additional information | construction of ferredoxin II mutants Q39R/S28E and D64N by site-directed mutagenesis, expression of recombinant wild-type and mutant ferredoxins of Equisetum arvense in Escherichia coli | Equisetum arvense |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | additional information | - |
additional information | kinetics | Equisetum arvense | |
1.18.1.2 | 0.0017 | - |
oxidized ferredoxin II | wild-type cofactor, pH 7.5, 25°C | Equisetum arvense | |
1.18.1.2 | 0.0053 | - |
oxidized ferredoxin II mutant D64N | pH 7.5, 25°C | Equisetum arvense | |
1.18.1.2 | 0.0114 | - |
oxidized ferredoxin I | wild-type cofactor, pH 7.5, 25°C | Equisetum arvense | |
1.18.1.2 | 0.0115 | - |
oxidized ferredoxin II mutant Q39R/S28E | pH 7.5, 25°C | Equisetum arvense |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | reduced ferredoxin + NADP+ | Equisetum arvense | - |
oxidized ferredoxin + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Equisetum arvense | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | oxidized ferredoxin I + NADPH + H+ | - |
Equisetum arvense | reduced ferredoxin I + NADP+ | - |
r | |
1.18.1.2 | oxidized ferredoxin II + NADPH + H+ | - |
Equisetum arvense | reduced ferredoxin II NADP+ | - |
r | |
1.18.1.2 | oxidized ferredoxin II mutant D64N + NADPH + H+ | - |
Equisetum arvense | reduced ferredoxin II mutant D64N + NADP+ | - |
r | |
1.18.1.2 | oxidized ferredoxin II mutant Q39R/S28E + NADPH + H+ | - |
Equisetum arvense | reduced ferredoxin mutant Q39R/S28E + NADP+ | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Equisetum arvense | oxidized ferredoxin + NADPH | - |
? | |
1.18.1.2 | reduced ferredoxin + NADP+ | recombinant ferredoxin I and II, and ferredoxin I mutants, the Fd I Q39R/S28E mutant lacks the Arg39-Glu28 residues being essential for efficient electron transfer between the cofactor and the enzyme | Equisetum arvense | oxidized ferredoxin + NADPH | - |
? | |
1.18.1.2 | reduced ferredoxin I + NADP+ | - |
Equisetum arvense | oxidized ferredoxin I + NADPH + H+ | - |
r | |
1.18.1.2 | reduced ferredoxin II + NADP+ | - |
Equisetum arvense | oxidized ferredoxin II + NADPH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | Fd-NADP+ reductase | - |
Equisetum arvense |
1.18.1.2 | Ferredoxin-NADP(+) reductase | - |
Equisetum arvense |
1.18.1.2 | FNR | - |
Equisetum arvense |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 25 | - |
assay at | Equisetum arvense |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 32.2 | - |
reduced ferredoxin II | wild-type cofactor, pH 7.5, 25°C | Equisetum arvense | |
1.18.1.2 | 36.8 | - |
reduced ferredoxin II | ferredoxin mutant D64N pH 7.5, 25°C | Equisetum arvense | |
1.18.1.2 | 147 | - |
reduced ferredoxin II | ferredoxin mutant Q39R/S28E, pH 7.5, 25°C | Equisetum arvense | |
1.18.1.2 | 177 | - |
reduced ferredoxin I | wild-type cofactor, pH 7.5, 25°C | Equisetum arvense |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 7.5 | - |
assay at | Equisetum arvense |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | Ferredoxin | [2Fe2S] cluster containing, ferredoxin I and II, and ferredoxin I mutants, the latter lacking the Arg39-Glu28 residues being essential for efficient electron transfer between the cofactor and the enzyme, the Fd I Q39R/S28E mutant is unstable and rapidly loose the [2Fe2S] cluster, the mutant shows 5.5fold reduced electron transfer rates compared to Fd I, ferredoxin II mutant D64N is about half as efficient as cofactor as the wild-type ferredoxin I and II are | Equisetum arvense |