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Literature summary extracted from

  • Yeo, C.C.; Wong, M.V.; Feng, Y.; Song, K.P.; Poh, C.L.
    Molecular characterization of an inducible gentisate 1,2-dioxygenase gene, xlnE, from Pseudomonas alcaligenes NCIMB 9867 (2003), Gene, 312, 239-248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expression in Escherichia coli Pseudomonas alcaligenes

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.4 additional information knockout mutant G56 Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2 Pseudomonas alcaligenes
-
 maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 Pseudomonas alcaligenes P25X
-
 maleylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Pseudomonas alcaligenes
-
-
-
1.13.11.4 Pseudomonas alcaligenes P25X
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.4 0.04
-
 23ºC, with gentistate as substrate, G56 mutant Pseudomonas alcaligenes
1.13.11.4 0.08
-
 23ºC, with lactate as substrate, wild-type enzyme Pseudomonas alcaligenes
1.13.11.4 0.09
-
 23ºC, with 2,5-xylenol as substrate, G56 mutant Pseudomonas alcaligenes
1.13.11.4 0.11
-
 23ºC, with 3-hydroxybenzoate as substrate, G56 mutant Pseudomonas alcaligenes
1.13.11.4 0.52
-
 23ºC, with gentistate as substrate, wild-type enzyme Pseudomonas alcaligenes
1.13.11.4 0.75
-
 23ºC, with 2,5-xylenol as substrate, wild-type enzyme Pseudomonas alcaligenes
1.13.11.4 0.81
-
 23ºC, with 3-hydroxybenzoate as substrate, wild-type enzyme Pseudomonas alcaligenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Pseudomonas alcaligenes maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Pseudomonas alcaligenes P25X maleylpyruvate
-
 ?
1.13.11.4 2,5-xylenol + O2
-
 Pseudomonas alcaligenes ?
-
 ?
1.13.11.4 2,5-xylenol + O2
-
 Pseudomonas alcaligenes P25X ?
-
 ?
1.13.11.4 3-hydroxybenzoate + O2
-
 Pseudomonas alcaligenes ?
-
 ?
1.13.11.4 3-hydroxybenzoate + O2
-
 Pseudomonas alcaligenes P25X ?
-
 ?
1.13.11.4 gentisate + O2
-
 Pseudomonas alcaligenes ?
-
 ?
1.13.11.4 gentisate + O2
-
 Pseudomonas alcaligenes P25X ?
-
 ?
1.13.11.4 lactate + O2
-
 Pseudomonas alcaligenes ?
-
 ?
1.13.11.4 lactate + O2
-
 Pseudomonas alcaligenes P25X ?
-
 ?