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Literature summary extracted from

  • Cui, Z.; Dunford, A.J.; Durrant, M.C.; Henderson, R.A.; Smith, B.E.
    Binding sites of nitrogenase: kinetic and theoretical studies of cyanide binding to extracted FeMo-cofactor derivatives (2003), Inorg. Chem., 42, 6252-6264.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 cyanide inhibits electron flow Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.6.1 additional information
-
 additional information reaction kinetics for the isolated MoFe protein in reaction with cyanide with ligands tert-butylcyanide, butyl isocyanide, or imidazole, bound to the cofactor protein, cyanide binding kinetics, stopped-flow measurements Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron dependent on, the enzyme complex contains a molybdenum-iron protein, and a dimeric iron protein Klebsiella pneumoniae
1.18.6.1 Molybdenum dependent on, the enzyme complex contains a molybdenum-iron protein harboring the active site Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O Klebsiella pneumoniae
-
 oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O Klebsiella pneumoniae M5a1
-
 oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Klebsiella pneumoniae
-
-
-
1.18.6.1 Klebsiella pneumoniae M5a1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 purification of the enzyme complex and isolation of the MoFe protein thereof Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate the active site is located on the MoFe protein, active site structure and substrate binding mechanism Klebsiella pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 acetylene + ?
-
 Klebsiella pneumoniae ethylene + ?
-
 ?
1.18.6.1 acetylene + ?
-
 Klebsiella pneumoniae M5a1 ethylene + ?
-
 ?
1.18.6.1 cyanide + ? cyanide favors the molydenum for binding Klebsiella pneumoniae ?
-
 ?
1.18.6.1 cyanide + ? cyanide favors the molydenum for binding Klebsiella pneumoniae M5a1 ?
-
 ?
1.18.6.1 cyclopropene + ?
-
 Klebsiella pneumoniae ?
-
 ?
1.18.6.1 cyclopropene + ?
-
 Klebsiella pneumoniae M5a1 ?
-
 ?
1.18.6.1 ethylene + ?
-
 Klebsiella pneumoniae ?
-
 ?
1.18.6.1 ethylene + ?
-
 Klebsiella pneumoniae M5a1 ?
-
 ?
1.18.6.1 methyl isocyanide + ?
-
 Klebsiella pneumoniae ?
-
 ?
1.18.6.1 N2O + ?
-
 Klebsiella pneumoniae ?
-
 ?
1.18.6.1 N3- + ?
-
 Klebsiella pneumoniae ?
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O
-
 Klebsiella pneumoniae oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir
1.18.6.1 reduced ferredoxin + H+ + N2 + ATP + H2O
-
 Klebsiella pneumoniae M5a1 oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.6.1 25
-
 assay at Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.6.1 8
-
 assay at Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.6.1 ATP
-
 Klebsiella pneumoniae
1.18.6.1 Ferredoxin
-
 Klebsiella pneumoniae
1.18.6.1 iron-molybdenum cofactor
-
 Klebsiella pneumoniae