EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.1 | cat1 and cat2, expression in Escherichia coli | Burkholderia sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.1 | catechol + O2 | Burkholderia sp. | both catechol dioxygenases, CatA1 and CatA2 are produced in benzoate-grown cells, CatA1 is undetectable when strain TH2 is grown on 2-chlorobenzoate or cis,cis-muconate. Production of CatA1 during growth on 2-chlorobenzoate or cis,cis-muconate is observed when cat2 genes are disrupted. The inducer of Cat1 is benzoate. cis,cis-Muconate or its metabolite acts as an inducer for CatA2. Genes are not indispensable for growth of strain TH2 on 2-chlorobenzoate, they are advantageous | cis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | Burkholderia sp. TH2 | both catechol dioxygenases, CatA1 and CatA2 are produced in benzoate-grown cells, CatA1 is undetectable when strain TH2 is grown on 2-chlorobenzoate or cis,cis-muconate. Production of CatA1 during growth on 2-chlorobenzoate or cis,cis-muconate is observed when cat2 genes are disrupted. The inducer of Cat1 is benzoate. cis,cis-Muconate or its metabolite acts as an inducer for CatA2. Genes are not indispensable for growth of strain TH2 on 2-chlorobenzoate, they are advantageous | cis,cis-muconate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.1 | Burkholderia sp. | Q8GAY6 | cat1 | - |
1.13.11.1 | Burkholderia sp. | Q8GAZ6 | cat2 | - |
1.13.11.1 | Burkholderia sp. TH2 | Q8GAY6 | cat1 | - |
1.13.11.1 | Burkholderia sp. TH2 | Q8GAZ6 | cat2 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.1 | 0.0948 | - |
CatA1 | Burkholderia sp. |
1.13.11.1 | 3.92 | - |
CatA2 | Burkholderia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.1 | 3-chlorocatechol + O2 | CatA1: 4.4% of the activity with catechol | Burkholderia sp. | 2-chloro-cis,cis-muconate | - |
? | |
1.13.11.1 | 3-chlorocatechol + O2 | CatA1: 4.4% of the activity with catechol | Burkholderia sp. TH2 | 2-chloro-cis,cis-muconate | - |
? | |
1.13.11.1 | 4-chlorocatechol + O2 | CatA1: 18.1% of the activity with catechol | Burkholderia sp. | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-chlorocatechol + O2 | CatA2: 2.5% of the activity with catechol | Burkholderia sp. | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-chlorocatechol + O2 | CatA1: 18.1% of the activity with catechol | Burkholderia sp. TH2 | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-chlorocatechol + O2 | CatA2: 2.5% of the activity with catechol | Burkholderia sp. TH2 | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | CatA1: 39.5% of the activity with catechol | Burkholderia sp. | 3-methyl-cis,cis-muconate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | CatA2: 13.1% of the activity with catechol | Burkholderia sp. | 3-methyl-cis,cis-muconate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | CatA1: 39.5% of the activity with catechol | Burkholderia sp. TH2 | 3-methyl-cis,cis-muconate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | CatA2: 13.1% of the activity with catechol | Burkholderia sp. TH2 | 3-methyl-cis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | - |
Burkholderia sp. | cis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | both catechol dioxygenases, CatA1 and CatA2 are produced in benzoate-grown cells, CatA1 is undetectable when strain TH2 is grown on 2-chlorobenzoate or cis,cis-muconate. Production of CatA1 during growth on 2-chlorobenzoate or cis,cis-muconate is observed when cat2 genes are disrupted. The inducer of Cat1 is benzoate. cis,cis-Muconate or its metabolite acts as an inducer for CatA2. Genes are not indispensable for growth of strain TH2 on 2-chlorobenzoate, they are advantageous | Burkholderia sp. | cis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | - |
Burkholderia sp. TH2 | cis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | both catechol dioxygenases, CatA1 and CatA2 are produced in benzoate-grown cells, CatA1 is undetectable when strain TH2 is grown on 2-chlorobenzoate or cis,cis-muconate. Production of CatA1 during growth on 2-chlorobenzoate or cis,cis-muconate is observed when cat2 genes are disrupted. The inducer of Cat1 is benzoate. cis,cis-Muconate or its metabolite acts as an inducer for CatA2. Genes are not indispensable for growth of strain TH2 on 2-chlorobenzoate, they are advantageous | Burkholderia sp. TH2 | cis,cis-muconate | - |
? | |
1.13.11.1 | additional information | CatA2: no activity with 3-chlorocatechol | Burkholderia sp. | ? | - |
? | |
1.13.11.1 | additional information | CatA2: no activity with 3-chlorocatechol | Burkholderia sp. TH2 | ? | - |
? |