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Literature summary extracted from

  • Nakahara, Y.; Okamoto, K.
    Unusual properties of the prespore-specific enzyme, UDP galactose:polysaccharide galactosyl transferase, of Dictyostelium discoideum (2004), J. Basic Microbiol., 44, 459-470.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.74 Polyethyleneglycol enhances activity Dictyostelium discoideum

General Stability

EC Number General Stability Organism
2.4.1.74 rapid loss of activity is prevented by the presence of albumin or Triton-X Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.74 ATP reversible, possibly regulates activity in vivo Dictyostelium discoideum
2.4.1.74 CuCl2 5-10 mM, more than 90% inhibition Dictyostelium discoideum
2.4.1.74 Lead acetate 5-10 mM, more than 90% inhibition Dictyostelium discoideum
2.4.1.74 p-chloromercuribenzoate 5-10 mM, more than 90% inhibition Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.74 0.006
-
 UDP-galactose
-
 Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.74 200000
-
 non-denaturing PAGE Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.74 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.74 partial Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.74 prespore specifically localized in. Activity sharply changes in response to differentiation and dedifferentiation Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.74 0.00000023
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.74 UDP-galactose + glycosaminoglycan
-
 Dictyostelium discoideum UDP + D-galactosylglycosaminoglycan
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.74 UDP galactose:polysaccharide galactosyl transferase
-
 Dictyostelium discoideum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.74
-
 21 reaction proceeds faster at 0°C than at 21°C Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.74 7.5
-
-
 Dictyostelium discoideum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.74 2
-
 ATP
-
 Dictyostelium discoideum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.74 Dictyostelium discoideum isoelectric focusing
-
 7