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Literature summary extracted from

  • Leimkuhler, S.; Hodson, R.; George, G.N.; Rajagopalan, K.V.
    Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans (2003), J. Biol. Chem., 278, 20802-20811.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.4 R135C mutation corresponding to human protein variant of a patient suffering from xanthinuria I. Mutation results in an active (alphabeta)2 heterotetrameric form besides an inactive alphabeta heterodimeric form missing the FeSI center Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Iron FeS center, EPR spectra, wild type 3.7 mol per subunit, active form of mutant R135C 2.8 mol per subunit, inactive form of mutant R135C 1.2 mol per subunit Rhodobacter capsulatus
1.17.1.4 Molybdenum active form of mutant R135C Moco content 97%, inactive form of mutant R135C Moco content 3.8% Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Bos taurus
-
-
-
1.17.1.4 Rhodobacter capsulatus
-
 expression in Escherichia coli
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 milk
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 acetaldehyde + 2,6-dichloroindophenol + H2O 0.1% of activity with xanthine Rhodobacter capsulatus ?
-
 ?
1.17.1.4 acetaldehyde + 2,6-dichloroindophenol + H2O 1.2% of activity with xanthine Bos taurus ?
-
 ?
1.17.1.4 glyceraldehyde + 2,6-dichloroindophenol + H2O 0.3% of activity with xanthine Rhodobacter capsulatus ?
-
 ?
1.17.1.4 glyceraldehyde + 2,6-dichloroindophenol + H2O 12.1% of activity with xanthine Bos taurus ?
-
 ?
1.17.1.4 hypoxanthine + NAD+ + H+ + O2- + H2O 12.4% of activity with xanthine Bos taurus xanthine + NADH + H2O2
-
 ?
1.17.1.4 hypoxanthine + NAD+ + H+ + O2- + H2O 19% of activity with xanthine Rhodobacter capsulatus xanthine + NADH + H2O2
-
 ?
1.17.1.4 pterin + 2,6-dichloroindophenol + H2O 22.7% of activity with xanthine Bos taurus ?
-
 ?
1.17.1.4 pterin + 2,6-dichloroindophenol + H2O 9.7% of activity with xanthine Rhodobacter capsulatus ?
-
 ?
1.17.1.4 purine + 2,6-dichloroindophenol + H2O 18.7% of activity with xanthine Bos taurus ?
-
 ?
1.17.1.4 purine + 2,6-dichloroindophenol + H2O 8.5% of activity with xanthine Rhodobacter capsulatus ?
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Bos taurus urate + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Rhodobacter capsulatus urate + NADH
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD 0.9 mol per subunit Rhodobacter capsulatus