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Literature summary extracted from

  • Zhang, G.; Dai, J.; Lu, Z.; Dunaway-Mariano, D.
    The phosphonopyruvate decarboxylase from Bacteroides fragilis (2003), J. Biol. Chem., 278, 41302-41308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.82 expression in Escherichia coli Bacteroides fragilis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.82 D258A 9% of wild-type kcat for 3-phosphonopyruvate Bacteroides fragilis
4.1.1.82 D260A 0.1% of wild-type kcat for 3-phosphonopyruvate Bacteroides fragilis
4.1.1.82 E213A 5% of wild-type kcat for 3-phosphonopyruvate Bacteroides fragilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.82 sulfopyruvate competitive inhibition Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.82 0.0032
-
 3-phosphonopyruvate 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine pyrophosphate Bacteroides fragilis
4.1.1.82 0.013
-
 Mn2+ 25°C, pH 7.3 Bacteroides fragilis
4.1.1.82 0.013
-
 thiamine diphosphate 25°C, pH 7.3 Bacteroides fragilis
4.1.1.82 0.078
-
 Ca2+ 25°C, pH 7.3 Bacteroides fragilis
4.1.1.82 0.082
-
 Mg2+ 25°C, pH 7.3 Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.82 Ca2+ required for activity Bacteroides fragilis
4.1.1.82 Mg2+ required for activity Bacteroides fragilis
4.1.1.82 Mn2+ required for activity Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.82 40000
-
 3 * 40000, SDS-PAGE Bacteroides fragilis
4.1.1.82 41199
-
 3 * 41199, MALDI-TOF mass spectroscopy Bacteroides fragilis
4.1.1.82 120000
-
 gel filtration Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.82 3-phosphonopyruvate Bacteroides fragilis
-
 2-phosphonoacetaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.82 Bacteroides fragilis Q9F768
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.82 recombinant Ppyr decarboxylase, ammonium sulfate, DEAE-Sepahrose, hydroxylapatite Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.82 3-phosphonopyruvate
-
 Bacteroides fragilis 2-phosphonoacetaldehyde + CO2
-
 ?
4.1.1.82 pyruvate 0.5% of 3-phosphonopyruvate kcat Bacteroides fragilis acetaldehyde + CO2
-
 ?
4.1.1.82 sulfopyruvate 0.5% of 3-phosphonopyruvate kcat Bacteroides fragilis sulfoacetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.82 trimer 3 * 40000, SDS-PAGE Bacteroides fragilis
4.1.1.82 trimer 3 * 41199, MALDI-TOF mass spectroscopy Bacteroides fragilis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.82 Ppyr decarboxylase
-
 Bacteroides fragilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.82 10.2
-
 3-phosphonopyruvate 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine diphosphate Bacteroides fragilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.82 thiamine diphosphate
-
 Bacteroides fragilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.82 0.2
-
 sulfopyruvate 25°C, pH 7.0 Bacteroides fragilis