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Literature summary extracted from

  • Gross, R.; Pisa, R.; Sanger, M.; Lancaster, C.R.; Simon, J.
    Characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of Wolinella succinogenes NiFe-hydrogenase (2004), J. Biol. Chem., 279, 274-281.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 expression of Strep-tagged enzyme Wolinella succinogenes

Protein Variants

EC Number Protein Variants Comment Organism
1.12.99.6 A204F no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 D100N no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 D88N no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 E94Q no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 G125L no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 H186A no acitivity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 H200A no acitivity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 H25A no acitivity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 H67A no acitivity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 M203I no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 N128D 5% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 P129A no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 Q131L 2% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 Y114F no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 Y127A 9% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes
1.12.99.6 Y127F no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 Y127H no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type Wolinella succinogenes
1.12.99.6 Y202F almost no activity with 2,3-dimethyl1,4-naphthoquinone Wolinella succinogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 membrane membrane-bound, enzyme is oriented to the periplasmic space Wolinella succinogenes 16020
-
1.12.99.6 periplasm
-
 Wolinella succinogenes
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe FeNi-hydrogenase Wolinella succinogenes
1.12.99.6 Ni FeNi-hydrogenase Wolinella succinogenes

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Wolinella succinogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 recombinant enzyme Wolinella succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + 2,3-dimethyl-1,4-naphthoquinone
-
 Wolinella succinogenes 2,3-dimethyl-naphthoquinol
-
 ?
1.12.99.6 H2 + oxidized benzyl viologen
-
 Wolinella succinogenes reduced benzyl viologen + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 trimer subunits HydA, HydB and HydC Wolinella succinogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.99.6 heme b involved in electron transport Wolinella succinogenes