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Literature summary extracted from

  • Jepson, B.J.N.; Anderson, L.J.; Rubio, L.M.; Taylor, C.J.; Butler, C.S.; Flores, E.; Herrero, A.; Butt, J.N.; Richardson, D.J.
    Tuning a nitrate reductase for function: the first spectropotentiometric characterization of a bacterial assimilatory nitrate reductase reveals novel redox properties (2004), J. Biol. Chem., 279, 32212-32218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.7.2 expression of His-tagged nitrate reductase in Escherichia coli Synechococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.2 azide competitive inhibition Synechococcus sp.
1.7.7.2 cyanide mixed inhibition, 50% inhibition at 0.01 mM Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.2 0.05
-
 nitrate 25°C, pH 8.0, electron donor methylviologen Synechococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.2 Iron nitrate reductase contains 1 [4Fe-4S] cluster Synechococcus sp.
1.7.7.2 Molybdenum
-
 Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.2 78000
-
 x * 78000, SDS-PAGE Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin Synechococcus sp.
-
 nitrite + H2O + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Synechococcus sp.
-
 PCC 7942
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2 recombinant nitrate reductase Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 chlorate + reduced methylviologen 6% of kcat with nitrate Synechococcus sp. chlorite + H2O + oxidized methylviologen
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced methylviologen
-
 Synechococcus sp. nitrite + H2O + oxidized methylviologen
-
 ?
1.7.7.2 selenate + reduced methylviologen 1% of kcat with nitrate Synechococcus sp. selenite + H2O + oxidized methylviologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.2 ? x * 78000, SDS-PAGE Synechococcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.7.7.2 narB
-
 Synechococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.7.2 80
-
 nitrate 25°C, pH 8.0, electron donor methylviologen Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.2 molybdopterin
-
 Synechococcus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.7.2 13
-
 azide 25°C, pH 8.0 Synechococcus sp.