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Literature summary extracted from

  • Grzyb, J.; Waloszek, A.; Latowski, D.; Wieckowski, S.
    Effect of cadmium on ferredoxin:NADP+ oxidoreductase activity (2004), J. Inorg. Biochem., 98, 1338-1346.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.2 Cd2+ 70% inhibition, uncompetitive to dibromothymoquinone, noncompetitive inhibition of NADPH oxidation, Zn2+ diminishes the inhibitory effect for dibromothymoquinone reduction, but enhances inhibition of ferricyanide reduction, inhibitory effect on ferricyanide reduction, but on dibromothymoquinone reduction, is abolished by addition of 2-mercaptoethanol or histidine, inhibition mechnanism, overview Spinacia oleracea
1.18.1.2 additional information histidine and 2-mercaptoethanol are not inhibitory Spinacia oleracea
1.18.1.2 Zn2+ 80% inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0031
-
 NADPH in presence of Cd2+ Spinacia oleracea
1.18.1.2 0.0058
-
 NADPH
-
 Spinacia oleracea
1.18.1.2 0.0119
-
 dibromothymoquinone
-
 Spinacia oleracea
1.18.1.2 0.0132
-
 dibromothymoquinone in presence of Cd2+ Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 chloroplast
-
 Spinacia oleracea 9507
-
1.18.1.2 thylakoid membrane tightly bound or loosely bound on the outer surface, 2 enzyme pools Spinacia oleracea 42651
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 35000
-
 native PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 reduced ferredoxin + NADP+ Spinacia oleracea terminal step in the non-cyclic photosynthetic electron transfer chain oxidized ferredoxin + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 from spinach leaves in several steps, to homogeneity Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.2 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 ferricyanide + NADPH i.e. K3Fe(CN)6, diaphorase activity Spinacia oleracea 2 ferrocyanide + NADP+ + H+
-
 ?
1.18.1.2 dibromothymoquinone + NADPH diaphorase activity Spinacia oleracea reduced dibromothymoquinone + NADP+
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Spinacia oleracea oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+ terminal step in the non-cyclic photosynthetic electron transfer chain Spinacia oleracea oxidized ferredoxin + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 35000, SDS-PAGE Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 FNR
-
 Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 12.5
-
 NADPH in presence of Cd2+ Spinacia oleracea
1.18.1.2 12.6
-
 dibromothymoquinone in presence of Cd2+ Spinacia oleracea
1.18.1.2 23.2
-
 NADPH
-
 Spinacia oleracea
1.18.1.2 25
-
 dibromothymoquinone
-
 Spinacia oleracea