EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.1 | gene glgP, DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Aquifex aeolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | additional information | - |
additional information | kinetics, forward and reverse reaction, recombinant enzyme | Aquifex aeolicus | |
2.4.1.1 | 0.17 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.18 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.19 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.28 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.3 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.3 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.38 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 0.55 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 1.8 | - |
maltotriose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 80000 | - |
x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
2.4.1.1 | 81981 | - |
x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | (1,4-alpha-D-glucosyl)n + phosphate | Aquifex aeolicus | polysaccharide substrate is glycogen, mobilization of glucose 1-phosphate as readily useable energy source | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Aquifex aeolicus | O66932 | gene glgP | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | recombinant His-tagged enzyme from Escherichia coli 6.3fold to homogeneity by heat treatment at 70°C and nickel affinity chromatography | Aquifex aeolicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | sequential random bi bi mechanism | Aquifex aeolicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 7.8 | - |
purified recombinant enzyme | Aquifex aeolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrate is glycogen, mobilization of glucose 1-phosphate as readily useable energy source | Aquifex aeolicus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrates are glycogen and starch, phosphorolysis is the preferred reaction | Aquifex aeolicus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | maltoheptaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltooctaose + phosphate | - |
r | |
2.4.1.1 | maltohexaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltoheptaose + phosphate | - |
r | |
2.4.1.1 | maltopentaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltohexaose + phosphate | - |
r | |
2.4.1.1 | maltotetraose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltopentaose + phosphate | - |
r | |
2.4.1.1 | maltotriose + alpha-D-glucose 1-phosphate | maltotriose is the smallest substrate accepted for the synthetic reaction direction, maltotetraose is the smallest substrate accepted for the phosphorolytic reaction direction | Aquifex aeolicus | maltotetraose + phosphate | - |
r | |
2.4.1.1 | additional information | maltotriose is the smallest substrate accepted for the synthetic reaction direction, maltotetraose is the smallest substrate accepted for the phosphorolytic reaction direction | Aquifex aeolicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | ? | x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.1 | glycogen phosphorylase | - |
Aquifex aeolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 100 | - |
recombinant enzyme | Aquifex aeolicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 90 | - |
84% remaining activity after 30 min, recombinant enzyme | Aquifex aeolicus |
2.4.1.1 | 100 | - |
66% remaining activity after 30 min, recombinant enzyme | Aquifex aeolicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | 0.76 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 2.2 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 2.3 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 2.7 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.4.1.1 | 4.1 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 4.4 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 5.4 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 6 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
2.4.1.1 | 6.8 | - |
maltotriose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 6.5 | - |
recombinant enzyme | Aquifex aeolicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 4 | 10 | recombinant enzyme, stable, 61% remaining activity at 70°C and pH 10.4 | Aquifex aeolicus |