Literature summary extracted from
Liu, D.Q.; Liu, H.; Gao, X.L.; Leak, D.J.; Zhou, N.Y.
Arg169 is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from Klebsiella pneumoniae M5a1 (2005), Microbiol. Res., 160, 53-59.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.13.24 |
gene mhbM, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha |
Klebsiella oxytoca |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.13.24 |
R169E |
site-directed mutagenesis, inactive mutant |
Klebsiella oxytoca |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.13.24 |
44000 |
- |
x * 44000, about, SDS-PAGE |
Klebsiella oxytoca |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.13.24 |
3-hydroxybenzoate + NADH + O2 |
Klebsiella oxytoca |
- |
2,5-dihydroxybenzoate + NAD+ + H2O |
i.e. gentisate |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.13.24 |
Klebsiella oxytoca |
Q5EXK1 |
gene mhbM |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.14.13.24 |
3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O |
Arg169 is essential for catalytic activity |
Klebsiella oxytoca |
|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.13.24 |
0.53 |
- |
recombinant wild-type enzyme |
Klebsiella oxytoca |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.13.24 |
3-hydroxybenzoate + NADH + O2 |
- |
Klebsiella oxytoca |
2,5-dihydroxybenzoate + NAD+ + H2O |
i.e. gentisate |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.13.24 |
? |
x * 44000, about, SDS-PAGE |
Klebsiella oxytoca |
1.14.13.24 |
More |
Ser166 and Arg169 form the highly conserved SXXR motif important for structure and function of the enzyme |
Klebsiella oxytoca |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.13.24 |
3-hydroxybenzoate 6-hydroxylase |
- |
Klebsiella oxytoca |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.13.24 |
30 |
- |
assay at |
Klebsiella oxytoca |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.13.24 |
8 |
- |
assay at |
Klebsiella oxytoca |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.13.24 |
FAD |
- |
Klebsiella oxytoca |
|
1.14.13.24 |
NADH |
- |
Klebsiella oxytoca |
|