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Literature summary extracted from
- Arg169 is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from Klebsiella pneumoniae M5a1 (2005), Microbiol. Res., 160, 53-59.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.24 | gene mhbM, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha | Klebsiella oxytoca |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.13.24 | R169E | site-directed mutagenesis, inactive mutant | Klebsiella oxytoca |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 44000 | - |
x * 44000, about, SDS-PAGE | Klebsiella oxytoca |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate + NADH + O2 | Klebsiella oxytoca | - |
2,5-dihydroxybenzoate + NAD+ + H2O | i.e. gentisate | ? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.24 | Klebsiella oxytoca | Q5EXK1 | gene mhbM | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O | Arg169 is essential for catalytic activity | Klebsiella oxytoca |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 0.53 | - |
recombinant wild-type enzyme | Klebsiella oxytoca |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate + NADH + O2 | - |
Klebsiella oxytoca | 2,5-dihydroxybenzoate + NAD+ + H2O | i.e. gentisate | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.24 | ? | x * 44000, about, SDS-PAGE | Klebsiella oxytoca |
| 1.14.13.24 | More | Ser166 and Arg169 form the highly conserved SXXR motif important for structure and function of the enzyme | Klebsiella oxytoca |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate 6-hydroxylase | - |
Klebsiella oxytoca |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 30 | - |
assay at | Klebsiella oxytoca |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 8 | - |
assay at | Klebsiella oxytoca |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.24 | FAD | - |
Klebsiella oxytoca | |
| 1.14.13.24 | NADH | - |
Klebsiella oxytoca | |
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Release 2023.1 (January 2023)
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