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Literature summary extracted from

  • Jeong, J.J.; Kim, J.H.; Kim, C.K.; Hwang, I.; Lee, K.
    3- and 4-alkylphenol degradation pathway in Pseudomonas sp. strain KL28: genetic organization of the lap gene cluster and substrate specificities of phenol hydroxylase and catechol 2,3-dioxygenase (2003), Microbiology, 149, 3265-3277.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Pseudomonas putida G7.C-1 and Escherichia coli DH5alpha Pseudomonas sp.
1.14.13.7
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 4-alkylcatechol + O2 Pseudomonas sp. initial step of long-chain alkylphenol cleavage pathway ?
-
 ?
1.13.11.2 4-alkylcatechol + O2 Pseudomonas sp. KL28 initial step of long-chain alkylphenol cleavage pathway ?
-
 ?
1.13.11.2 catechol + O2 Pseudomonas sp. involved in metabolism of aromatic compounds 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas sp. KL28 involved in metabolism of aromatic compounds 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp. Q7WYF5
-
-
1.13.11.2 Pseudomonas sp. KL28 Q7WYF5
-
-
1.14.13.7 Pseudomonas sp.
-
-
-
1.14.13.7 Pseudomonas sp. KL28
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 10
-
 with 2,3-dihydroxybiphenyl, pH 7.5, 28°C Pseudomonas sp.
1.13.11.2 20
-
 with catechol, pH 7.5, 28°C Pseudomonas sp.
1.13.11.2 68
-
 with 3-methylcatechol, pH 7.5, 28°C Pseudomonas sp.
1.13.11.2 320
-
 with 4-ethylcatechol, pH 7.5, 28°C Pseudomonas sp.
1.13.11.2 593
-
 with 4-methylcatechol, pH 7.5, 28°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 2,3-dihydroxybiphenyl + O2 poor substrate Pseudomonas sp. ?
-
 ?
1.13.11.2 2,3-dihydroxybiphenyl + O2 poor substrate Pseudomonas sp. KL28 ?
-
 ?
1.13.11.2 3-methylcatechol + O2 poor substrate Pseudomonas sp. 2-hydroxy-3-methylmuconate semialdehyde
-
 ?
1.13.11.2 4-alkylcatechol + O2 initial step of long-chain alkylphenol cleavage pathway Pseudomonas sp. ?
-
 ?
1.13.11.2 4-alkylcatechol + O2 alkyl chain length C1-C5 is accepted as substrate Pseudomonas sp. ?
-
 ?
1.13.11.2 4-alkylcatechol + O2 initial step of long-chain alkylphenol cleavage pathway Pseudomonas sp. KL28 ?
-
 ?
1.13.11.2 4-alkylcatechol + O2 alkyl chain length C1-C5 is accepted as substrate Pseudomonas sp. KL28 ?
-
 ?
1.13.11.2 4-ethylcatechol + O2 effective substrate Pseudomonas sp. 4-ethyl-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-methylcatechol + O2 very effective substrate Pseudomonas sp. 2-hydroxy-4-methylmuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 poor substrate Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 involved in metabolism of aromatic compounds Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 poor substrate Pseudomonas sp. KL28 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 involved in metabolism of aromatic compounds Pseudomonas sp. KL28 2-hydroxymuconate semialdehyde
-
 ?
1.14.13.7 3-ethylphenol + NADH + H+ + O2
-
 Pseudomonas sp. 4-ethylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 3-ethylphenol + NADH + H+ + O2
-
 Pseudomonas sp. KL28 4-ethylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 4-ethylphenol + NADH + H+ + O2
-
 Pseudomonas sp. 4-ethylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 4-ethylphenol + NADH + H+ + O2
-
 Pseudomonas sp. KL28 4-ethylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 4-propylphenol + NADH + H+ + O2 low activity Pseudomonas sp. 4-propylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 4-propylphenol + NADH + H+ + O2 low activity Pseudomonas sp. KL28 4-propylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 m-cresol + NADH + H+ + O2
-
 Pseudomonas sp. 4-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 m-cresol + NADH + H+ + O2
-
 Pseudomonas sp. KL28 4-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 o-cresol + NADH + H+ + O2 low activity Pseudomonas sp. 3-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 p-cresol + NADH + H+ + O2
-
 Pseudomonas sp. 4-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2
-
 Pseudomonas sp. KL28 catechol + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 Mph
-
 Pseudomonas sp.
1.14.13.7 multicomponent phenol hydroxylase
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADH
-
 Pseudomonas sp.