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Literature summary extracted from

  • Kurkela, M.; Morsky, S.; Hirvonen, J.; Kostiainen, R.; Finel, M.
    An active and water-soluble truncation mutant of the human UDP-glucuronosyltransferase 1A9 (2004), Mol. Pharmacol., 65, 826-831.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 expression of isozyme UGT1A9 lacking 45 residues of the C-terminus including the trans-membrane helix, enzyme mutant is His6-tagged at the shortened C-terminus, detergent-free expression as soluble protein in insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.17 additional information either detergent binding to the transmembrane helix within the full-length isozyme UGT1A9 or removal of this helix affect the interaction of the enzyme with its aglycone substrate in a similar manner Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 Triton X-100 inhibition of recombinant soluble isozyme UGT1A9 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 additional information
-
 additional information kinetics Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 membrane integral Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens O60656 isozyme UGT1A9; isozyme UGT1A9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 recombinant His-tagged isozyme UGT1A9 mutant from insect cells by nickel affinity chromatogaphy Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 additional information substrate specificities of wild-type and recombinant soluble mutant isozyme UGT1A9 Homo sapiens ?
-
 ?
2.4.1.17 UDP-glucuronate + 1-naphthol
-
 Homo sapiens UDP + 1-naphthol beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + 4-nitrophenol
-
 Homo sapiens UDP + 4-nitrophenol beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + entacapone
-
 Homo sapiens UDP + entacapone beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + scopoletin
-
 Homo sapiens UDP + scopoletin beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + umbelliferone
-
 Homo sapiens UDP + umbelliferone beta-D-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 More
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 UDP-glucuronosyltransferase
-
 Homo sapiens
2.4.1.17 UGT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.4
-
 assay at Homo sapiens