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Literature summary extracted from

  • Niggeweg, R.; Michael, A.J.; Martin, C.
    Engineering plants with increased levels of the antioxidant chlorogenic acid (2004), Nat. Biotechnol., 22, 746-754.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.99 agriculture overexpression of the enzyme in tomato causes plants to accumulate higher levels of chlorogenic acid, with no side-effects on the levels of other soluble phenolics. Plants show improved antioxidant capacity and resistance to infection by bacterial pathogens. Tomatoes with elevated levels of chlorogenic acid could be used in foods with specific benefits for human health Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.99 expression in Escherichia coli as fusion protein with 15 amino acid S-TAG Solanum lycopersicum
2.3.1.99 expression in Escherichia coli as fusion protein with S-TAG Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.871
-
 quinate
-
 Nicotiana tabacum
2.3.1.99 11.82
-
 shikimate
-
 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.99 additional information Nicotiana tabacum the enzyme is required for the synthesis of chlorogenic acid ?
-
 ?
2.3.1.99 additional information Solanum lycopersicum the enzyme is required for the synthesis of chlorogenic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Nicotiana tabacum Q70G33
-
-
2.3.1.99 Solanum lycopersicum Q70G32
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99
-
 Nicotiana tabacum
2.3.1.99
-
 Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 1.08
-
-
 Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 caffeoyl-CoA + quinate
-
 Nicotiana tabacum CoA + 5-O-caffeoylquinate
-
 ?
2.3.1.99 caffeoyl-CoA + quinate
-
 Solanum lycopersicum CoA + 5-O-caffeoylquinate
-
 ?
2.3.1.99 caffeoyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.99 caffeoyl-CoA + shikimate
-
 Solanum lycopersicum CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.99 additional information the enzyme is required for the synthesis of chlorogenic acid Nicotiana tabacum ?
-
 ?
2.3.1.99 additional information the enzyme is required for the synthesis of chlorogenic acid Solanum lycopersicum ?
-
 ?
2.3.1.99 p-coumaroyl-CoA + quinate
-
 Nicotiana tabacum CoA + 5-O-(p-coumaroyl)quinate
-
 ?
2.3.1.99 p-coumaroyl-CoA + quinate
-
 Solanum lycopersicum CoA + 5-O-(p-coumaroyl)quinate
-
 ?
2.3.1.99 p-coumaroyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-(p-coumaroyl)shikimate
-
 ?
2.3.1.99 p-coumaroyl-CoA + shikimate
-
 Solanum lycopersicum CoA + 5-O-(p-coumaroyl)shikimate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.99 HQT
-
 Nicotiana tabacum
2.3.1.99 HQT
-
 Solanum lycopersicum