EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.9 | gene APR1, expression of the holoenzyme APR1p in Escherichia coli strain BL21(DE3), separate expression in Escherichia coli strain BL21(DE3) of amino acid residues 73-327, forming the R-domain, and of residues 328-465, forming the C-domain, the domains alone are inactive, but mixing of both can partially restore activity | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.9 | additional information | gene APR1, the holoenzyme APR1p as well as the C-domain alone can complement the cysteine auxotrophy of an Escherichia coli cysH mutant strain, substituting for glutaredoxin, if the mutant is capable of producing glutathione, cysH encodes the 5'-phosphoadenylylsulfate reductase in Escherichia coli | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.9 | glutathione | slightly inhibitory at high concentrations | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.9 | 0.6 | - |
glutathione | holoenzyme APR1p with substrate 5'-adenylyl sulfate, pH 8.5, 30°C | Arabidopsis thaliana | |
1.8.4.9 | 1.12 | - |
glutathione | about, holoenzyme APR1p and C-domain with substrates hydroxyethyldisulfide, or cystine, pH 8.0, 24°C | Arabidopsis thaliana | |
1.8.4.9 | 1.15 | - |
glutathione | about, holoenzyme APR1p and C-domain with substrate dehydroascorbate, pH 6.9, 24°C | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.9 | additional information | Arabidopsis thaliana | enzyme is involved in sulfate assimilation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.9 | Arabidopsis thaliana | P92979 | APR1; 3 genes APR1, APR2, and APR3, 3 isozymes APR1p, APR2p, and APR3p | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.9 | recombinant holoenzyme APR1p, C-domain, and R-domain from Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.4.9 | additional information | - |
- |
Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.9 | 5'-adenylyl sulfate + glutathione | catalyzed by the holoenzyme APR1p, not by the C-domain | Arabidopsis thaliana | AMP + sulfite + glutathione disulfide | - |
? | |
1.8.4.9 | cystine + glutathione | catalyzed by the holoenzyme APR1p and the C-domain | Arabidopsis thaliana | cysteine + glutathione disulfide | - |
? | |
1.8.4.9 | dehydroascorbate + glutathione | catalyzed by the holoenzyme APR1p and the C-domain | Arabidopsis thaliana | ascorbate + glutathione disulfide | - |
? | |
1.8.4.9 | hydroxyethyldisulfide + glutathione | catalyzed by the holoenzyme APR1p and the C-domain | Arabidopsis thaliana | ? | - |
? | |
1.8.4.9 | insulin disulfide + glutathione | catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain | Arabidopsis thaliana | insulin + glutathione disulfide | - |
? | |
1.8.4.9 | additional information | enzyme is involved in sulfate assimilation | Arabidopsis thaliana | ? | - |
? | |
1.8.4.9 | oxidized ribonucleotide reductase + glutathione | catalyzed by the C-domain, not by the holoenzyme APR1p or the R-domain | Arabidopsis thaliana | reduced ribonucleotide reductase + glutathione disulfide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.4.9 | More | amino acid residues 73-327 form the R-domain which is homologous to microbial 5'-phosphoadenylylsulfate reductase, and of residues 328-465 form the C-domain which is homologous to thioredoxin, the domains alone are inactive, but mixing of both can partially rstore activity | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.9 | 5'-adenylylsulfate reductase | - |
Arabidopsis thaliana |
1.8.4.9 | APR1p | - |
Arabidopsis thaliana |
1.8.4.9 | APS reductase | - |
Arabidopsis thaliana |
1.8.4.9 | More | enzyme belongs to the thioredoxin superfamily | Arabidopsis thaliana |
1.8.4.9 | plant-type 5'-adenylylsulfate reductase | - |
Arabidopsis thaliana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.9 | 24 | 30 | - |
Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.9 | 6.5 | 8.5 | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.9 | glutathione | dependent on, specific cofactor for the carboxyterminal glutathione-dependent reductase domain of the enzyme, can be exchanged for equally active DTT, glutathione is slightly inhibitory at high concentrations | Arabidopsis thaliana | |
1.8.4.9 | additional information | DTT can be exchanged for glutathione, equally active | Arabidopsis thaliana | |
1.8.4.9 | additional information | no activity with 2-mercaptoethanol, gamma-glutamylcysteine, or cysteine | Arabidopsis thaliana |