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Literature summary extracted from

  • Sobrado, V.R.; Montemartini-Kalisz, M.; Kalisz, H.M.; De La Fuente, M.C.; Hecht, H.J.; Nowicki, C.
    Involvement of conserved asparagine and arginine residues from the N-terminal region in the catalytic mechanism of rat liver and Trypanosoma cruzi tyrosine aminotransferases (2003), Protein Sci., 12, 1039-1050.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.5 N17S increase in Km-values, substantial decrease in kcat-values Trypanosoma cruzi
2.6.1.5 N54S reduced enzymic activity Rattus norvegicus
2.6.1.5 R20A increase in Km-values, substantial decrease in kcat-values Trypanosoma cruzi
2.6.1.5 R315K retains enzymic activity Rattus norvegicus
2.6.1.5 R417Q no enzymic activity Rattus norvegicus
2.6.1.5 R57A no enzymic activity Rattus norvegicus
2.6.1.5 R57Q no enzymic activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.3
-
 2-oxoisocaproate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.35
-
 2-oxoglutarate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 0.4
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 0.6
-
 pyruvate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.6
-
 pyruvate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.8
-
 pyruvate wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.9
-
 L-alanine wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 1.2
-
 L-tyrosine wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 1.2
-
 p-hydroxyphenylpyruvate wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 1.2
-
 L-alanine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 1.2
-
 L-alanine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 1.2
-
 p-hydroxyphenylpyruvate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 1.3
-
 p-hydroxyphenylpyruvate mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 1.9
-
 L-glutamate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 2.1
-
 L-tyrosine wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 2.2
-
 L-glutamate wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 2.3
-
 L-tyrosine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 2.3
-
 L-tyrosine mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 2.4
-
 L-tyrosine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 2.8
-
 2-oxoisocaproate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 3.5
-
 2-oxoglutarate mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 4.6
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 5.3
-
 L-tyrosine mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 8.8
-
 L-glutamate mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 9.6
-
 2-oxoglutarate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 14.8
-
 2-oxoglutarate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 20.4
-
 2-oxoisocaproate wild-type, pH 7.5, 37°C Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Rattus norvegicus P04694
-
-
2.6.1.5 Trypanosoma cruzi P33447
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-alanine + 2-oxoglutarate
-
 Trypanosoma cruzi pyruvate + L-glutamate
-
 ?
2.6.1.5 L-alanine + 2-oxoisocaproate
-
 Trypanosoma cruzi pyruvate + 2-aminoisocaproate
-
 ?
2.6.1.5 L-glutamate + 4-hydroxyphenylpyruvate
-
 Rattus norvegicus 2-oxoglutarate + L-tyrosine
-
 ?
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Rattus norvegicus 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.5 L-tyrosine + pyruvate
-
 Trypanosoma cruzi 4-hydroxyphenylpyruvate + L-alanine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.5 0.35
-
 L-alanine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.4
-
 2-oxoisocaproate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.5
-
 pyruvate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.5
-
 2-oxoglutarate mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 0.7
-
 L-tyrosine mutant R20A, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 1.5
-
 pyruvate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 1.8
-
 L-tyrosine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 3.8
-
 L-alanine mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 4.2
-
 2-oxoglutarate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 5.9
-
 p-hydroxyphenylpyruvate mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 6.6
-
 L-glutamate mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 9.3
-
 2-oxoisocaproate mutant N17S, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 21.8
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 23.5
-
 L-alanine wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 34
-
 2-oxoisocaproate wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 44.6
-
 L-tyrosine wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 47.4
-
 pyruvate wild-type, pH 7.5, 37°C Trypanosoma cruzi
2.6.1.5 116
-
 2-oxoglutarate mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 132
-
 L-tyrosine mutant N54S, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 155
-
 p-hydroxyphenylpyruvate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 165
-
 p-hydroxyphenylpyruvate wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 199
-
 L-glutamate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 210
-
 L-glutamate wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 490
-
 2-oxoglutarate mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 510
-
 L-tyrosine mutant R315K, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 566
-
 2-oxoglutarate wild-type, pH 7.5, 37°C Rattus norvegicus
2.6.1.5 576
-
 L-tyrosine wild-type, pH 7.5, 37°C Rattus norvegicus