Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakuraba, H.; Kawakami, R.; Ohshima, T.
    First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization (2005), Appl. Environ. Microbiol., 71, 4352-4358.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23
-
 Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.3
-
 NAD+ cosubstrate polyP27, pH 6.8, 70°C Pyrococcus horikoshii
2.7.1.23 0.4
-
 NAD+ cosubstrate ATP, pH 6.8, 70°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 CaCl2 5 mM, substrate polyP27, 57% activity, substrate ATP, 65% activity Pyrococcus horikoshii
2.7.1.23 CuCl2 5 mM, substrate polyP27, 100% activity, substrate ATP, 62% activity Pyrococcus horikoshii
2.7.1.23 MgCl2 5 mM, substrate polyP27, 100% activity, substrate ATP, 100% activity Pyrococcus horikoshii
2.7.1.23 MnCl2 5 mM, substrate polyP27, 100% activity, substrate ATP, 62% activity Pyrococcus horikoshii
2.7.1.23 NiCl2 5 mM, substrate polyP27, 80% activity, substrate ATP, 36% activity Pyrococcus horikoshii
2.7.1.23 ZnCl2 5 mM, substrate polyP27, 56% activity, substrate ATP, 78% activity Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 37000
-
 4 * 37000, SDS-PAGE Pyrococcus horikoshii
2.7.1.23 145000
-
 gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Pyrococcus horikoshii O58801
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23 recombinant enzyme Pyrococcus horikoshii

Storage Stability

EC Number Storage Stability Organism
2.7.1.23 precipitation during dialysis against phosphate buffer without NaCl and imidazole, storage in 0.5 M NaCl and 0.5 M imidazole at 4°C for up to 2 months without loss of activity Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+ 75% of the activity with polyP27 Pyrococcus horikoshii ADP + NADP+
-
 ?
2.7.1.23 ATP + NADH
-
 Pyrococcus horikoshii ADP + NADPH
-
 ?
2.7.1.23 CTP + NAD+ 76% of the activity with polyP27 Pyrococcus horikoshii CDP + NADP+
-
 ?
2.7.1.23 GTP + NAD+ 111% of the activity with polyP27 Pyrococcus horikoshii GDP + NADP+
-
 ?
2.7.1.23 ITP + NAD+ 89% of the activity with polyP27 Pyrococcus horikoshii IDP + NADP+
-
 ?
2.7.1.23 polyphosphate + NAD+ activity with polyphosphates in decreasing order: polyP27, polyP32, polyP18, polyP46, polyP62, polyP5, no activity with orthophosphate or diphosphate Pyrococcus horikoshii (phosphate)n-1 + NADP+
-
 ?
2.7.1.23 TTP + NAD+ 13% of the activity with polyP27 Pyrococcus horikoshii TDP + NADP+
-
 ?
2.7.1.23 UTP + NAD+ 94% of the activity with polyP27 Pyrococcus horikoshii UDP + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 tetramer 4 * 37000, SDS-PAGE Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.23 70
-
 60 min, stable in pH-range 4.0-10.5 Pyrococcus horikoshii
2.7.1.23 95
-
 10 min, stable Pyrococcus horikoshii
2.7.1.23 100
-
 10 min, 20% residual activity Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 6.8
-
-
 Pyrococcus horikoshii