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Literature summary extracted from

  • Kaper, T.; Talik, B.; Ettema, T.J.; Bos, H.; van der Maarel, M.J.; Dijkhuizen, L.
    Amylomaltase of Pyrobaculum aerophilum IM2 produces thermoreversible starch gels (2005), Appl. Environ. Microbiol., 71, 5098-5106.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.25 nutrition acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties. This thermoreversible gel has potential applications in the food industry Pyrobaculum aerophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expression in Escherichia coli Pyrobaculum aerophilum

General Stability

EC Number General Stability Organism
2.4.1.25 dithiothreitol decreases thermal stability of the enzyme Pyrobaculum aerophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 acarbose
-
 Pyrobaculum aerophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 1.5
-
 maltoheptaose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 2.1
-
 maltotetraose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 3.2
-
 maltohexaose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 3.7
-
 maltotriose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 6.9
-
 maltopentaose pH 6.7, 80°C Pyrobaculum aerophilum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Pyrobaculum aerophilum
-
 IM2
-
2.4.1.25 Pyrobaculum aerophilum IM2
-
 IM2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 recombinant enzyme Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltoheptaose + maltoheptaose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltoheptaose + maltoheptaose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltohexaose + maltohexaose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltohexaose + maltohexaose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotetraose + maltotetraose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotetraose + maltotetraose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Pyrobaculum aerophilum D-glucose + maltopentaose
-
 ?
2.4.1.25 additional information acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties Pyrobaculum aerophilum ?
-
 ?
2.4.1.25 additional information acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties Pyrobaculum aerophilum IM2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 amylmaltase
-
 Pyrobaculum aerophilum
2.4.1.25 PyAMase
-
 Pyrobaculum aerophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 95
-
-
 Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 87
-
 half-life: 506 min Pyrobaculum aerophilum
2.4.1.25 89
-
 half-life: 114 min Pyrobaculum aerophilum
2.4.1.25 95
-
 half-life: 107 min Pyrobaculum aerophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 8.7
-
 maltoheptaose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 8.9
-
 maltohexaose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 24.8
-
 maltopentaose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 51.2
-
 maltotetraose pH 6.7, 80°C Pyrobaculum aerophilum
2.4.1.25 115
-
 maltotriose pH 6.7, 80°C Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6.7
-
-
 Pyrobaculum aerophilum