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Literature summary extracted from

  • Enayetallah, A.E.; Grant, D.F.
    Effects of human soluble epoxide hydrolase polymorphisms on isoprenoid phosphate hydrolysis (2006), Biochem. Biophys. Res. Commun., 341, 254-260.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.10 medicine kinetic analysis of the effects of human enzyme polymorphisms on the N-terminal phosphatase activity of soluble epoxide hydrolase activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.76 expression of enzyme in insect cells using the baculovirus infection system Rattus norvegicus
3.1.3.76 expression of His-tagged wild-type and mutant enzymes in insect cells using the baculovirus infection system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.76 C154Y the mutant shows similar activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens
3.1.3.76 R103C the mutant shows increased activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens
3.1.3.76 R103C/R287Q the mutant shows similar activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens
3.1.3.76 R287Q the mutant shows increased activity for isoprenoid phosphate compared to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.76 additional information no inhibition of the phosphatase activity by 1-cyclohexyl-3-dodecyl urea at 0.1 mM Homo sapiens
3.1.3.76 orthovanadate 65% inhibition of the phosphatase activity at 0.1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.76 0.024
-
farnesyl phosphate recombinant enzyme Homo sapiens
3.1.3.76 0.033
-
geranylgeranyl diphosphate recombinant enzyme Homo sapiens
3.1.3.76 0.084
-
farnesyl diphosphate recombinant enzyme Homo sapiens
3.1.3.76 0.124
-
geranyl phosphate recombinant enzyme Homo sapiens
3.1.3.76 1.746
-
geranylgeranyl phosphate recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.76 cytosol
-
Homo sapiens 5829
-
3.1.3.76 cytosol
-
Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.76 Mg2+ dependent on Rattus norvegicus
3.1.3.76 Mg2+ dependent on, maximal activity at 0.1 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.76 Homo sapiens
-
-
-
3.1.3.76 Rattus norvegicus
-
-
-
3.3.2.10 Homo sapiens
-
bifunctional epoxide hydrolase/phosphatase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.76 recombinant enzyme by S-benzyl affinity chromatography with 4-fluorochalcone oxide as eluent Rattus norvegicus
3.1.3.76 recombinant His-tagged wild-type and mutant enzymes from insect cells by nickel affinity chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.76 (9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Homo sapiens
3.1.3.76 (9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.76 liver
-
Homo sapiens
-
3.1.3.76 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.76 0.03
-
purified recombinant enzyme, substrate isopentenyl phosphate Homo sapiens
3.1.3.76 0.25
-
purified recombinant enzyme, substrate geranyl phosphate Homo sapiens
3.1.3.76 0.49
-
purified recombinant enzyme, substrate farnesyl phosphate Homo sapiens
3.1.3.76 0.53
-
purified recombinant enzyme, substrate geranylgeranyl phosphate Homo sapiens
3.1.3.76 0.8
-
purified recombinant enzyme, substrate geranyl phosphate Rattus norvegicus
3.1.3.76 0.85
-
purified recombinant enzyme, substrate isopentenyl phosphate Rattus norvegicus
3.1.3.76 0.99
-
purified recombinant enzyme, substrate farnesyl phosphate Rattus norvegicus
3.1.3.76 1.05
-
purified recombinant enzyme, substrate geranylgeranyl phosphate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.76 farnesyl diphosphate + H2O
-
Homo sapiens farnesol + phosphate
-
?
3.1.3.76 farnesyl phosphate + H2O
-
Homo sapiens farnesol + phosphate
-
?
3.1.3.76 farnesyl phosphate + H2O
-
Rattus norvegicus farnesol + phosphate
-
?
3.1.3.76 geranyl diphosphate + H2O
-
Homo sapiens geraniol + phosphate
-
?
3.1.3.76 geranyl phosphate + H2O
-
Homo sapiens geraniol + phosphate
-
?
3.1.3.76 geranyl phosphate + H2O
-
Rattus norvegicus geraniol + phosphate
-
?
3.1.3.76 geranylgeranyl diphosphate + H2O
-
Homo sapiens geranylgeraniol + phosphate
-
?
3.1.3.76 geranylgeranyl phosphate + H2O
-
Homo sapiens geranylgeraniol + phosphate
-
?
3.1.3.76 geranylgeranyl phosphate + H2O
-
Rattus norvegicus geranylgeraniol + phosphate
-
?
3.1.3.76 isopentenyl diphosphate + H2O
-
Homo sapiens isopentenol + phosphate
-
?
3.1.3.76 isopentenyl phosphate + H2O
-
Homo sapiens isopentenol + phosphate
-
?
3.1.3.76 isopentenyl phosphate + H2O
-
Rattus norvegicus isopentenol + phosphate
-
?
3.3.2.10 additional information kinetic analysis of the effects of human enzyme polymorphisms on the N-terminal phosphatase activity of soluble epoxide hydrolase activity Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.76 More c.f. EC 3.3.2.10 Homo sapiens
3.1.3.76 More c.f. EC 3.3.2.10 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.76 0.13
-
geranylgeranyl diphosphate recombinant enzyme Homo sapiens
3.1.3.76 0.25
-
farnesyl diphosphate recombinant enzyme Homo sapiens
3.1.3.76 0.51
-
geranyl phosphate recombinant enzyme Homo sapiens
3.1.3.76 0.71
-
farnesyl phosphate recombinant enzyme Homo sapiens
3.1.3.76 1.85
-
geranylgeranyl phosphate recombinant enzyme Homo sapiens