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Literature summary extracted from

  • Yamamoto, S.; Uchimura, K.; Wakayama, M.; Tachiki, T.
    Purification and characterization of glutamine synthetase of Pseudomonas taetrolens Y-30: an enzyme usable for production of theanine by coupling with the alcoholic fermentation system of baker's yeast (2004), Biosci. Biotechnol. Biochem., 68, 1888-1897.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.1.2 synthesis isolated glutamine synthetase forms theanine in the mixture with the yeast fermentation system, production in high concentrations can be achieved Pseudomonas taetrolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 additional information
-
additional information
-
Pseudomonas taetrolens
6.3.1.2 0.56
-
NH4+ pH 8.0, 30 mM Mg2+ Pseudomonas taetrolens
6.3.1.2 0.59
-
NH4+ pH 7.5, 3 mM MN2+ Pseudomonas taetrolens
6.3.1.2 1.3
-
ATP pH 11.0, 30 mM Mg2+ Pseudomonas taetrolens
6.3.1.2 1.4
-
ATP pH 8.0, 3 mM Mn2+ Pseudomonas taetrolens
6.3.1.2 1.4
-
ATP pH 8.5, 30 mM Mg2+ Pseudomonas taetrolens
6.3.1.2 1.5
-
ATP pH 7.5, 3 mM MN2+ Pseudomonas taetrolens
6.3.1.2 1.5
-
ATP pH 8.5, 3 mM Mn2+ Pseudomonas taetrolens
6.3.1.2 2
-
ATP pH 8.0, 30 mM Mg2+ Pseudomonas taetrolens
6.3.1.2 20
-
methylamine pH 8.0, 3 mM Mn2+ Pseudomonas taetrolens
6.3.1.2 100
-
methylamine pH 8.5, 30 mM Mg2+ Pseudomonas taetrolens
6.3.1.2 500
-
ethylamine pH 8.5, 3 mM Mn2+ Pseudomonas taetrolens
6.3.1.2 700
-
ethylamine pH 11.0, 30 mM Mg2+ Pseudomonas taetrolens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ activates, optimum: 15 mM Pseudomonas taetrolens
6.3.1.2 Mn2+ activates with 11% of the efficiency of the activation with Mg2+, optimum: 3-4 mM Pseudomonas taetrolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 55000
-
12 * 55000, SDS-PAGE Pseudomonas taetrolens
6.3.1.2 66000
-
gel filtration Pseudomonas taetrolens

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Pseudomonas taetrolens
-
srain Y-30
-
6.3.1.2 Pseudomonas taetrolens Y-30
-
srain Y-30
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
Pseudomonas taetrolens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 1.17
-
-
Pseudomonas taetrolens

Storage Stability

EC Number Storage Stability Organism
6.3.1.2 -20°C, stable for two months Pseudomonas taetrolens
6.3.1.2 5°C, about 95% of the initial activity is maintained for 30 days in a state of ammonium sulfate precipitate (70% saturation) Pseudomonas taetrolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + ethylamine 1% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+ Pseudomonas taetrolens ADP + phosphate + L-Gln + ?
-
?
6.3.1.2 ATP + L-Glu + ethylamine 1% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+ Pseudomonas taetrolens Y-30 ADP + phosphate + L-Gln + ?
-
?
6.3.1.2 ATP + L-Glu + hydroxylamine 32% of the activity with NH4+ Pseudomonas taetrolens ADP + phosphate + L-Gln + ?
-
?
6.3.1.2 ATP + L-Glu + hydroxylamine 32% of the activity with NH4+ Pseudomonas taetrolens Y-30 ADP + phosphate + L-Gln + ?
-
?
6.3.1.2 ATP + L-Glu + methylamine 7% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+ Pseudomonas taetrolens ADP + phosphate + L-Gln + ?
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Pseudomonas taetrolens ADP + phosphate + L-Gln
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Pseudomonas taetrolens Y-30 ADP + phosphate + L-Gln
-
?
6.3.1.2 GTP + L-Glu + NH4+ 26% of the activity with ATP Pseudomonas taetrolens GDP + phosphate + L-Gln
-
?
6.3.1.2 GTP + L-Glu + NH4+ 26% of the activity with ATP Pseudomonas taetrolens Y-30 GDP + phosphate + L-Gln
-
?
6.3.1.2 ITP + L-Glu + NH4+ 18% of the activity with ATP Pseudomonas taetrolens IDP + phosphate + L-Gln
-
?
6.3.1.2 ITP + L-Glu + NH4+ 18% of the activity with ATP Pseudomonas taetrolens Y-30 IDP + phosphate + L-Gln
-
?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer 12 * 55000, SDS-PAGE Pseudomonas taetrolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 55
-
10 min, stable Pseudomonas taetrolens
6.3.1.2 65
-
10 min, 40% loss of activity Pseudomonas taetrolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.5
-
activated with 3 mM Mn2+ Pseudomonas taetrolens
6.3.1.2 8
-
activated by 30 mM Mg2+ Pseudomonas taetrolens
6.3.1.2 8.5
-
Mn2+-dependent reaction with ethylamine Pseudomonas taetrolens
6.3.1.2 11
-
Mg2+-dependent reaction with ethylamine Pseudomonas taetrolens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.1.2 6
-
stable Pseudomonas taetrolens