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Literature summary extracted from

  • Pesaresi, A.; Devescovi, G.; Lamba, D.; Venturi, V.; Degrassi, G.
    Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1 (2005), Curr. Microbiol., 50, 102-109.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Pseudomonas aeruginosa
3.1.1.1 expression in Escherichia coli Pseudomonas aeruginosa PAO1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Co2+
-
Pseudomonas aeruginosa PAO1
3.1.1.1 CoCl2 1mM, 34% residual activity Pseudomonas aeruginosa
3.1.1.1 Cu2+
-
Pseudomonas aeruginosa PAO1
3.1.1.1 CuCl2 1 mM, 59% residual activity Pseudomonas aeruginosa
3.1.1.1 dithiothreitol 1 mM, 58% residual activity Pseudomonas aeruginosa
3.1.1.1 DTT 1 mM, 42% inhibition Pseudomonas aeruginosa PAO1
3.1.1.1 Fe2+
-
Pseudomonas aeruginosa PAO1
3.1.1.1 Fe3+
-
Pseudomonas aeruginosa PAO1
3.1.1.1 FeCl2 1 mM, 40% residual activity Pseudomonas aeruginosa
3.1.1.1 FeCl3 1 mM, 38% residual activity Pseudomonas aeruginosa
3.1.1.1 additional information not inhibitory: EDTA Pseudomonas aeruginosa
3.1.1.1 additional information no inhibition by EDTA Pseudomonas aeruginosa PAO1
3.1.1.1 Ni2+
-
Pseudomonas aeruginosa PAO1
3.1.1.1 NiCl2 1 mM, 35% residual activity Pseudomonas aeruginosa
3.1.1.1 phenylmethylsulfonyl fluoride 0.1 mM, 48% residual activity Pseudomonas aeruginosa
3.1.1.1 phenylmethylsulfonyl fluoride complete inhibition above 1 mM Pseudomonas aeruginosa PAO1
3.1.1.1 Zn2+
-
Pseudomonas aeruginosa PAO1
3.1.1.1 ZnCl2 1 mM, 36% residual activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.43
-
p-nitrophenyl acetate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 0.43
-
4-nitrophenyl acetate pH 9.0, 25°C Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 MgCl2 slight activation Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 19300
-
gel filtration Pseudomonas aeruginosa
3.1.1.1 19300
-
gel filtration Pseudomonas aeruginosa PAO1
3.1.1.1 24000
-
1 * 24000, SDS-PAGE Pseudomonas aeruginosa PAO1
3.1.1.1 24691
-
1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS Pseudomonas aeruginosa
3.1.1.1 28000
-
1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pseudomonas aeruginosa
-
-
-
3.1.1.1 Pseudomonas aeruginosa PAO1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant Pseudomonas aeruginosa PAO1
3.1.1.1 recombinant protein Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 7310
-
25°C, pH 7.0 Pseudomonas aeruginosa PAO1
3.1.1.1 7310
-
pH 9.0, 25°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 1-naphthyl acetate + H2O
-
Pseudomonas aeruginosa 1-naphthol + acetate
-
?
3.1.1.1 1-naphthyl butanoate + H2O 54% of the activity with 1-naphthyl acetate Pseudomonas aeruginosa 1-naphthol + butanoate
-
?
3.1.1.1 1-naphthyl caprylate + H2O 14% of the activity with 1-naphthyl acetate Pseudomonas aeruginosa 1-naphthol + caprylate
-
?
3.1.1.1 1-naphthyl laurate + H2O 9% of the activity with 1-naphthyl acetate Pseudomonas aeruginosa 1-naphthol + laurate
-
?
3.1.1.1 1-naphthyl oleate + H2O 2% of the activity with 1-naphthyl acetate Pseudomonas aeruginosa 1-naphthol + oleate
-
?
3.1.1.1 4-methylumbelliferyl acetate + H2O
-
Pseudomonas aeruginosa 4-methylumbelliferone + acetate
-
?
3.1.1.1 4-methylumbelliferyl acetate + H2O
-
Pseudomonas aeruginosa PAO1 4-methylumbelliferone + acetate
-
?
3.1.1.1 4-nitrophenyl acetate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + acetate
-
?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + butanoate
-
?
3.1.1.1 alpha-naphthyl acetate + H2O
-
Pseudomonas aeruginosa PAO1 alpha-naphthol + acetate
-
?
3.1.1.1 alpha-naphthyl butyrate + H2O
-
Pseudomonas aeruginosa PAO1 alpha-naphthol + butyrate
-
?
3.1.1.1 carboxyfluorescein diacetate + H2O
-
Pseudomonas aeruginosa carboxyfluorescein + acetate
-
?
3.1.1.1 carboxyfluorescein diacetate + H2O
-
Pseudomonas aeruginosa PAO1 ?
-
?
3.1.1.1 fluorescein diacetate + H2O
-
Pseudomonas aeruginosa PAO1 ?
-
?
3.1.1.1 fluorescein diacetate + H2O
-
Pseudomonas aeruginosa fluorescein + acetate
-
?
3.1.1.1 p-nitrophenyl acetate + H2O
-
Pseudomonas aeruginosa PAO1 p-nitrophenol + acetate
-
?
3.1.1.1 p-nitrophenyl butyrate
-
Pseudomonas aeruginosa PAO1 p-nitrophenol + butyrate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 24000, SDS-PAGE Pseudomonas aeruginosa PAO1
3.1.1.1 monomer 1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 55
-
-
Pseudomonas aeruginosa
3.1.1.1 55
-
-
Pseudomonas aeruginosa PAO1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 45 65 45°C: about 60% of maximal activity, 65°C: about 50% of maximal activity Pseudomonas aeruginosa PAO1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 50
-
pH 9, 2 h, stable up to Pseudomonas aeruginosa PAO1
3.1.1.1 50
-
pH 9.0, 2 h, stable up to 50°C Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 170
-
fluorescein diacetate
-
Pseudomonas aeruginosa
3.1.1.1 170
-
fluorescein diacetate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 1600
-
1-naphthyl butanoate
-
Pseudomonas aeruginosa
3.1.1.1 1600
-
alpha-naphthyl butyrate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 1700
-
p-nitrophenyl butyrate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 1700
-
4-nitrophenyl butanoate
-
Pseudomonas aeruginosa
3.1.1.1 2800
-
carboxyfluorescein diacetate
-
Pseudomonas aeruginosa
3.1.1.1 2800
-
carboxyfluorescein diacetate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 3000
-
Alpha-naphthyl acetate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 3000
-
1-naphthyl acetate
-
Pseudomonas aeruginosa
3.1.1.1 6400
-
4-nitrophenyl acetate
-
Pseudomonas aeruginosa
3.1.1.1 6400
-
p-nitrophenyl acetate
-
Pseudomonas aeruginosa PAO1
3.1.1.1 8500
-
4-yethylumbelliferyl acetate
-
Pseudomonas aeruginosa
3.1.1.1 8500
-
4-yethylumbelliferyl acetate
-
Pseudomonas aeruginosa PAO1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9
-
-
Pseudomonas aeruginosa
3.1.1.1 9
-
-
Pseudomonas aeruginosa PAO1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 8 10 about 40% of maximal activity at pH 8.0 and 10.0 Pseudomonas aeruginosa PAO1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 4 8 stable Pseudomonas aeruginosa PAO1

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Pseudomonas aeruginosa calculated
-
5
3.1.1.1 Pseudomonas aeruginosa isoelectric focusing
-
5.2
3.1.1.1 Pseudomonas aeruginosa PAO1 isoelectric focusing, pH-range 3-10
-
5.2