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Literature summary extracted from

  • Piskur, J.; Sandrini, M.P.B.; Knecht, W.; Munch-Petersen, B.
    Animal deoxyribonucleoside kinases: 'forward' and 'retrograde' evolution of their substrate specificity (2004), FEBS Lett., 560, 3-6.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.74 A100V/R104M/D133A site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, mutant shows 30fold increased kcat for deoxycytidine compared to the wild-type enzyme Homo sapiens
2.7.1.74 R104M/D133A site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, the mutant gains the ability to phosphorylate deoxythymidine Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 cytosol
-
 Homo sapiens 5829
-
2.7.1.74 cytosol
-
 Mus musculus 5829
-
2.7.1.113 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+
-
 Homo sapiens
2.7.1.74 Mg2+
-
 Mus musculus
2.7.1.113 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine Homo sapiens
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyadenosine Mus musculus
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine Homo sapiens
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine Mus musculus
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine Homo sapiens
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine Mus musculus
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 additional information Homo sapiens the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview ?
-
 ?
2.7.1.74 additional information Mus musculus the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview ?
-
 ?
2.7.1.113 ATP + 2'-deoxyadenosine Homo sapiens
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyguanosine Homo sapiens
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyinosine Homo sapiens
-
 ADP + 2'-deoxyinosine 5'-phosphate
-
 ?
2.7.1.113 additional information Homo sapiens the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview ?
-
 ?
2.7.1.145 additional information Drosophila melanogaster enzyme shows broad substrate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens P27707
-
-
2.7.1.74 Mus musculus P43346
-
-
2.7.1.113 Homo sapiens Q16854
-
-
2.7.1.145 Drosophila melanogaster Q9XZT6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyadenosine
-
 Mus musculus ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Mus musculus ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine
-
 Mus musculus ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.74 additional information the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview Homo sapiens ?
-
 ?
2.7.1.74 additional information the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview Mus musculus ?
-
 ?
2.7.1.113 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyinosine
-
 Homo sapiens ADP + 2'-deoxyinosine 5'-phosphate
-
 ?
2.7.1.113 additional information the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview Homo sapiens ?
-
 ?
2.7.1.145 additional information enzyme shows broad substrate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information enzyme shows broad substrate specificity involving V84, M88, and A110 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens
2.7.1.74 dCK
-
 Mus musculus
2.7.1.113 DGK
-
 Homo sapiens
2.7.1.145 dNK
-
 Drosophila melanogaster
2.7.1.145 More the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.74 ATP
-
 Homo sapiens
2.7.1.74 ATP
-
 Mus musculus
2.7.1.113 ATP
-
 Homo sapiens