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Literature summary extracted from

  • Herr, D.R.; Fyrst, H.; Creason, M.B.; Phan, V.H.; Saba, J.D.; Harris, G.L.
    Characterization of the Drosophila sphingosine kinases and requirement for Sk2 in normal reproductive function (2004), J. Biol. Chem., 279, 12685-12694.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91 gene Sk1, DNA and amino acid sequence determination and analysis, 2 splicing variants, location on the X chromosome, functional overexpression of Sk1 in the yeast strain JSK398 Drosophila melanogaster
2.7.1.91 gene Sk2, DNA and amino acid sequence determination and analysis, a single intron, location on chromosome 3, functional overexpression of Sk2 in the yeast strain JSK398 Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.91 additional information complementation of yeast enzyme-deficient mutant strain by both Sk1 and Sk2 Drosophila melanogaster
2.7.1.91 additional information complementation of yeast enzyme-deficient mutant strain by both Sk1 and Sk2, a Drosophila melanogaster null Sk2 transposon insertion mutant shows elevated long chain base levels, impaired flight performance, and diminished ovulation, phenotype analysis Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.91 cytosol isozymes Sk1 and Sk2, predominantly Drosophila melanogaster 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.91 ATP + dihydrosphingosine Drosophila melanogaster C14 and C16 dihydrosphingosine substrates ADP + dihydrosphingosine 1-phosphate
-
?
2.7.1.91 ATP + sphingosine Drosophila melanogaster C14 and C16 sphingosine substrates ADP + sphingosine 1-phosphate
-
?
2.7.1.91 additional information Drosophila melanogaster requirement for Sk2 in normal reproductive function, product S1P signaling promotes cell proliferation, survival, and migration, enzyme reduces cellular levels of sphingosine and ceramide, sphingolipid metabolism, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Drosophila melanogaster Q9VYY8 isozyme Sk1; 2 isozymes Sk1 and Sk2 encoded by 2 genes Sk1 and Sk2
-
2.7.1.91 Drosophila melanogaster Q9VZW0 isozyme Sk2; 2 isozymes Sk1 and Sk2 encoded by 2 genes Sk1 and Sk2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 embryo expression pattern of Sk1 during embryogenesis Drosophila melanogaster
-
2.7.1.91 embryo expression pattern of Sk2 during embryogenesis Drosophila melanogaster
-
2.7.1.91 additional information the 2 genes Sk1 and Sk2 show overlapping but distinct temporal and spatial expression patterns Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + dihydrosphingosine
-
Drosophila melanogaster ADP + dihydrosphingosine 1-phosphate
-
?
2.7.1.91 ATP + dihydrosphingosine C14 and C16 dihydrosphingosine substrates Drosophila melanogaster ADP + dihydrosphingosine 1-phosphate
-
?
2.7.1.91 ATP + sphingosine
-
Drosophila melanogaster ADP + sphingosine 1-phosphate
-
?
2.7.1.91 ATP + sphingosine C14 and C16 sphingosine substrates Drosophila melanogaster ADP + sphingosine 1-phosphate
-
?
2.7.1.91 additional information requirement for Sk2 in normal reproductive function, product S1P signaling promotes cell proliferation, survival, and migration, enzyme reduces cellular levels of sphingosine and ceramide, sphingolipid metabolism, overview Drosophila melanogaster ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 SK
-
Drosophila melanogaster
2.7.1.91 sphinganine kinase
-
Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.91 37
-
assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 7.2
-
assay at Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.91 ATP
-
Drosophila melanogaster