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Literature summary extracted from

  • Goyer, A.; Collakova, E.; Diaz de la Garza, R.; Quinlivan, E.P.; Williamson, J.; Gregory, J.F., 3rd; Shachar-Hill, Y.; Hanson, A.D.
    5-Formyltetrahydrofolate is an inhibitory but well tolerated metabolite in Arabidopsis leaves (2005), J. Biol. Chem., 280, 26137-26142.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.2 insertional mutant DNA sequence determination and analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 additional information 5-CHO-THF cycloligase mutant, doubled leaf 5-formyltetrahydrofolate level, little impact on SHMT activity, but glycine content of mutant leaves is 19fold higher than the wild-type, also a small accumulation of serine in the mutant relative to the wild-type Arabidopsis thaliana
6.3.3.2 additional information insertional mutation of the gene encoding the enzyme, At5g13050 or 28D07, reduces the growth rate of the mutant plants by 20% and delayes flowering by 1 week, and leads to accumulation of 5-formyltetrahydrofolate and glycine in leaves under photorespiratory conditions, effect of 5-formyltetrahydrofolate feeding, overview Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 5-formyltetrahydrofolate can inhibit SHMT in vivo and thereby influence glycine pool size, can accumulate glycine in both wild-type and 5-CHO-THF cycloligase mutant Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 mitochondrion
-
 Arabidopsis thaliana 5739
-
6.3.3.2 mitochondrion
-
 Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate Arabidopsis thaliana recycling reaction ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Arabidopsis thaliana
-
-
-
6.3.3.2 Arabidopsis thaliana
-
 ecotype Columbia
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 leaf
-
 Arabidopsis thaliana
-
6.3.3.2 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Arabidopsis thaliana glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Arabidopsis thaliana ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate recycling reaction Arabidopsis thaliana ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Arabidopsis thaliana
2.1.2.1 SHMT
-
 Arabidopsis thaliana
6.3.3.2 5,10-Methenyltetrahydrofolate synthetase
-
 Arabidopsis thaliana
6.3.3.2 5-CHO-THF cycloligase
-
 Arabidopsis thaliana
6.3.3.2 5-FCL
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.3.2 ATP
-
 Arabidopsis thaliana