Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Christensen, K.E.; Patel, H.; Kuzmanov, U.; Mejia, N.R.; MacKenzie, R.E.
    Disruption of the mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria (2005), J. Biol. Chem., 280, 7597-7602.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.3 DNA sequence determination and analysis Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.3 additional information construction of a DCS disruption knockout mutant fibroblast SF6 cell line lacking the cytoplasmic isozyme but expressing active mitochondrial isozyme from a disruption mutant embryonic stem cell line Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.3 cytosol trifunctional cytosolic isozyme Mus musculus 5829
-
6.3.4.3 mitochondrion mitochondrial monofunctional isozyme Mus musculus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Mus musculus flow of one-carbon units through cytoplasmic and mitochondrial folate pathways, overview ADP + phosphate + 10-formyltetrahydrofolate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Mus musculus Q922D8 DCS, complete gene and exon 27; gene mthfd1 encodes the cytosolic isozyme which is a trifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase enzyme DCS, a mitochondrial monofunctional 10-formyltetrahydrofolate synthetase isozyme is encoded by a different gene
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.3 embryo
-
 Mus musculus
-
6.3.4.3 embryonic stem cell
-
 Mus musculus
-
6.3.4.3 fibroblast
-
 Mus musculus
-
6.3.4.3 fibroblast SF6 cell, immortalized fibroblast with disrupted gene encoding the trifunctional cytosolic DCS Mus musculus
-
6.3.4.3 additional information isozyme expression analysis and growth rates of cell lines, overview Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.3 additional information
-
 mitochondrial isozyme activity in different cell lines, overview Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Mus musculus ADP + phosphate + 10-formyltetrahydrofolate
-
 r
6.3.4.3 ATP + formate + tetrahydrofolate flow of one-carbon units through cytoplasmic and mitochondrial folate pathways, overview Mus musculus ADP + phosphate + 10-formyltetrahydrofolate
-
 r

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 10-Formyltetrahydrofolate synthetase
-
 Mus musculus
6.3.4.3 10-formylTHF synthetase
-
 Mus musculus
6.3.4.3 DCS
-
 Mus musculus
6.3.4.3 Formyltetrahydrofolate synthetase
-
 Mus musculus
6.3.4.3 methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase enzyme
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.3 ADP
-
 Mus musculus
6.3.4.3 ATP
-
 Mus musculus