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Literature summary extracted from

  • Lopez-Gallego, F.; Betancor, L.; Hidalgo, A.; Mateo, C.; Guisan, J.M.; Fernandez-Lafuente, R.
    Optimization of an industrial biocatalyst of glutaryl acylase: Stabilization of the enzyme by multipoint covalent attachment onto new amino-epoxy Sepabeads (2004), J. Biotechnol., 111, 219-227.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.1.93 45°C, pH 7.0, complete inactivation of soluble enzyme after 2 h, complete inactivation of enzyme immobilized on CNBr activated agarose and of enzyme immobilized on glyoxyl agarose, 35% inactivation of enzyme immobilized on amino-epoxy Sepabeads, 60% inactivation of enzyme immobilized on glutaraldehyde activated agarose, 70% inactivation of enzyme immobilized on epoxy Sepabeads unidentified

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 unidentified
-
 purchased from Roche
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.93 additional information
-
 it is possible to immobilize up to 85 IU/g at 25°C that corresponds to 200 IU/g at 37°C unidentified

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 42
-
 enzyme immobilized on cyanogen bromide agarose unidentified
3.5.1.93 52
-
 enzyme immobilized on amino-epoxy Sepabead derivatives unidentified

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.93 24 47 24°C: about 50% of maximal activity, 47°C: about 60% of maximal activity, enzyme immobilized on cyanogen bromide agarose unidentified
3.5.1.93 47 57 47°C: about 50% of maximal activity, 57°C: about 60% of maximal activity, enzyme immobilized on amino-epoxy Sepabead derivatives unidentified

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.93 45
-
 pH 7.0, complete inactivation of soluble enzyme after 2 h, complete inactivation of enzyme immobilized on CNBr activated agarose and of enzyme immobilized on glyoxyl agarose, 35% inactivation of enzyme immobilized on amino-epoxy Sepabeads, 60% inactivation of enzyme immobilized on glutaraldehyde activated agarose, 70% inactivation of enzyme immobilized on epoxy Sepabeads unidentified

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 7.5
-
 enzyme immobilized on cyanogen bromide agarose, enzyme immobilized on amino-epoxy Sepabead derivatives and enzyme and soluble enzyme unidentified

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.93 6 9 pH 6.0: about 40% of maximal activity of enzyme immobilized on cyanogen bromide agarose, about 55% of maximal activity of soluble enzyme and enzyme immobilized on amino-epoxy Sepabead derivatives, pH 9.0: about 90% of maximal activity of soluble enzyme, about 70% of maximal activity of cyanogen bromide agarose derivatives unidentified