Any feedback?
Literature summary extracted from
- Improvement of nikkomycin production by enhanced copy of sanU and sanV in Streptomyces ansochromogenes and characterization of a novel glutamate mutase encoded by sanU and sanV (2005), Metab. Eng., 7, 165-173.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.1 | individual expression of SanU and SanV in Escherichia coli | Streptomyces ansochromogenes |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.1 | 5.2 | - |
L-threo-3-methylaspartate | 37°C | Streptomyces ansochromogenes |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.1 | Co2+ | 0.1 mol per mol of subunit SanU | Streptomyces ansochromogenes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | 18000 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
| 5.4.99.1 | 18370 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
| 5.4.99.1 | 47850 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
| 5.4.99.1 | 48000 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.1 | Streptomyces ansochromogenes | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 5.4.99.1 | additional information | sulfhydryl compounds are not essential for maintaining active SanU, but for activity of reassembled enzyme compolex | Streptomyces ansochromogenes |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.1 | after individual expression of SanU and SanV in Escherichia coli | Streptomyces ansochromogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.1 | L-glutamate | - |
Streptomyces ansochromogenes | L-threo-3-methylaspartate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.1 | ? | x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
| 5.4.99.1 | More | no enzymic activity for subunit SanU or SanV alone, enzymic activity results after mixing both subunits plus coenzyme B12, reversible and dynamical equilibrium between SanU and SanV in formation of a possibly tetrameric complex | Streptomyces ansochromogenes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.1 | SanU | - |
Streptomyces ansochromogenes |
| 5.4.99.1 | SanV | - |
Streptomyces ansochromogenes |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | 35 | 42 | - |
Streptomyces ansochromogenes |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.1 | 7.5 | 8.5 | - |
Streptomyces ansochromogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.1 | coenzyme B12 | binds to subunit SanU with about 0.1 mol Co per mol SanU | Streptomyces ansochromogenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
