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Literature summary extracted from

  • Rein, U.; Gueta, R.; Denger, K.; Ruff, J.; Hollemeyer, K.; Cook, A.M.
    Dissimilation of cysteate via 3-sulfolactate sulfo-lyase and a sulfate exporter in Paracoccus pantotrophus NKNCYSA (2005), Microbiology, 151, 737-747.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.24 additional information the enzyme is strongly inducible Paracoccus pantotrophus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.24 gene suyAB, DNA and amino acid sequence determination and analysis Paracoccus pantotrophus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.24 EDTA 70% inhibition at 1 mM Paracoccus pantotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.24 Co2+ can substitute for Fe2+ at 2mM Paracoccus pantotrophus
4.4.1.24 Fe2+ required for activity Paracoccus pantotrophus
4.4.1.24 Mn2+ can substitute for Fe2+ at 2mM Paracoccus pantotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.24 8000
-
 x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE Paracoccus pantotrophus
4.4.1.24 42000
-
 x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.24 3-sulfolactate Paracoccus pantotrophus desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview pyruvate + bisulfite
-
 ?
4.4.1.24 additional information Paracoccus pantotrophus activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.337 Paracoccus pantotrophus
-
-
-
1.1.1.337 Paracoccus pantotrophus NKNCYSA / DSM 12449
-
-
-
4.4.1.24 Paracoccus pantotrophus Q58Y44 strongly inducible enzyme, gene suyAB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.24 from cysteate-grown cells by anion exchange chromatography and hydrophobic interaction chromatography, to near homogeneity Paracoccus pantotrophus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.24 (2R)-3-sulfolactate = pyruvate + hydrogensulfite putative reaction mechanism Paracoccus pantotrophus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.24 culture condition:(R)-cysteate-grown cell i.e. 2-amino-3-sulfopropionate, is the sole carbon and energy source of the organism with either nitrate or molecular oxygen as terminal electron acceptor Paracoccus pantotrophus
-
4.4.1.24 culture condition:sulfolactate-grown cell
-
 Paracoccus pantotrophus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.337 2.52
-
 pH 7.5, 30°C Paracoccus pantotrophus
4.4.1.24 0.3
-
 purified enzyme Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.337 3-sulfopyruvate + NADH + H+
-
 Paracoccus pantotrophus 3-sulfolactate + NAD+
-
 ?
1.1.1.337 3-sulfopyruvate + NADH + H+
-
 Paracoccus pantotrophus NKNCYSA / DSM 12449 3-sulfolactate + NAD+
-
 ?
4.4.1.24 3-sulfolactate
-
 Paracoccus pantotrophus pyruvate + bisulfite
-
 ?
4.4.1.24 3-sulfolactate desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview Paracoccus pantotrophus pyruvate + bisulfite
-
 ?
4.4.1.24 additional information activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization Paracoccus pantotrophus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.24 ? x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE Paracoccus pantotrophus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.337 sulfolactate dehydrogenase
-
 Paracoccus pantotrophus
4.4.1.24 3-sulfolactate sulfo-lyase
-
 Paracoccus pantotrophus
4.4.1.24 More the enzyme belongs to the altronate dehydratase family Paracoccus pantotrophus
4.4.1.24 Suy
-
 Paracoccus pantotrophus

General Information

EC Number General Information Comment Organism
1.1.1.337 physiological function enzyme is part of the degradative pathway from (R)-cysteate to pyruvate and sulfate Paracoccus pantotrophus