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Literature summary extracted from

  • Wischgoll, S.; Heintz, D.; Peters, F.; Erxleben, A.; Sarnighausen, E.; Reski, R.; Van Dorsselaer, A.; Boll, M.
    Gene clusters involved in anaerobic benzoate degradation of Geobacter metallireducens (2005), Mol. Microbiol., 58, 1238-1252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.25 overexpression in Escherichia coli Geobacter metallireducens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.25 2-Hydroxybenzoate competitive Geobacter metallireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.03
-
 benzoate
-
 Geobacter metallireducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 60000
-
 gel filtration Geobacter metallireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.25 ATP + benzoate + CoA Geobacter metallireducens the enzyme catalyzes the initial step of benzoate metabolism AMP + diphosphate + benzoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Geobacter metallireducens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25 His-tagged recombinant enzyme Geobacter metallireducens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 16
-
-
 Geobacter metallireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-aminobenzoate + CoA 3% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 92% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 23% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA 98% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Geobacter metallireducens AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA the enzyme catalyzes the initial step of benzoate metabolism Geobacter metallireducens AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + cyclohex-1-ene-1-carboxylate + CoA 13% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA
-
 ?
6.2.1.25 ATP + cyclohex-3-ene-1-carboxylate + CoA 40% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA
-
 ?
6.2.1.25 ATP + cyclohexanecarboxylate + CoA 7% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + cyclohexanecarboxyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 monomer 1 * 60000, SDS-PAGE Geobacter metallireducens

Synonyms

EC Number Synonyms Comment Organism
6.2.1.25 BamY
-
 Geobacter metallireducens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.25 0.016
-
 2-Hydroxybenzoate
-
 Geobacter metallireducens