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Literature summary extracted from

  • Boehlein, S.K.; Sewell, A.K.; Cross, J.; Stewart, J.D.; Hannah, L.C.
    Purification and characterization of adenosine diphosphate glucose pyrophosphorylase from maize/potato mosaics (2005), Plant Physiol., 138, 1552-1562.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate 10 mM, 16fold stimulation, enzyme from maize endosperm Zea mays
2.7.7.27 D-fructose 25 mM, 1.3fold stimulation, enzyme from maize endosperm Zea mays
2.7.7.27 D-fructose 6-phosphate 25 mM, 15fold stimulation, enzyme from maize endosperm Zea mays
2.7.7.27 D-glucose 6-phosphate 25 mM, 12fold stimulation, enzyme from maize endosperm Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber, expression in Escherichia coli Zea mays
2.7.7.27 mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber, expression in Escherichia coli Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 additional information mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber. Km for ATP and alpha-D-glucose 1-phosphate do not differ significantly for the mosaic enzymes in the presence of 3-phosphoglycerate. 2fold increase in Km for ATP when the potato small subunit is combined with the maize large subunit (Pss/Mls). Interestingly, the turnover number is increased for all mosaics Zea mays
2.7.7.27 additional information mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber. Km for ATP and alpha-D-glucose 1-phosphate do not differ significantly for the mosaic enzymes in the presence of 3-phosphoglycerate. 2fold increase in Km for ATP when the potato small subunit is combined with the maize large subunit (Pss/Mls). Interestingly, the turnover number is increased for all mosaics Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information KM-values for mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Zea mays
2.7.7.27 additional information
-
 additional information KM-values for mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Solanum tuberosum
2.7.7.27 0.06
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, enzyme from Zea mays endosperm Zea mays
2.7.7.27 0.12
-
 ATP pH 7.4, 37°C, enzyme from Zea mays endosperm Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Zea mays rate-limiting step in starch biosynthesis diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Solanum tuberosum rate-limiting step in starch biosynthesis diphosphate + ADP-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum tuberosum
-
-
-
2.7.7.27 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 recombinant mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Zea mays
2.7.7.27 recombinant mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Zea mays
-
2.7.7.27 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 41.1
-
 recombinant enzyme from maize endosperm Zea mays

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 -80°C, majority of activity is retained for several months, mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Solanum tuberosum
2.7.7.27 -80°C, majority of activity is retained for several months, recombinant enzyme from maize and mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate rate-limiting step in starch biosynthesis Zea mays diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate rate-limiting step in starch biosynthesis Solanum tuberosum diphosphate + ADP-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 adenosine diphosphate glucose pyrophosphorylase
-
 Zea mays
2.7.7.27 adenosine diphosphate glucose pyrophosphorylase
-
 Solanum tuberosum
2.7.7.27 AGPase
-
 Zea mays
2.7.7.27 AGPase
-
 Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information turnover numbers for mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Zea mays
2.7.7.27 additional information
-
 additional information turnover numbers for mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber Solanum tuberosum
2.7.7.27 85
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C, enzyme from Zea mays endosperm Zea mays
2.7.7.27 98
-
 ATP pH 7.4, 37°C, enzyme from Zea mays endosperm Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 2.96
-
 phosphate pH 7.4, 37°C, enzyme from maize endosperm Zea mays