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Literature summary extracted from

  • Nagashima, S.; Inagaki, R.; Kubo, A.; Hirotani, M.; Yoshikawa, T.
    cDNA cloning and expression of isoflavonoid-specific glucosyltransferase from Glycyrrhiza echinata cell-suspension cultures (2004), Planta, 218, 456-459.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.170 additional information the enzyme is stress-inducible, transient induction by cell suspension culture treatment with yeast extract Glycyrrhiza echinata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.170 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Glycyrrhiza echinata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.170 additional information the enzyme is stress-inducible, no inhibition by baicalein, kaempferol, naringenin, apigenin, quercetin, scopoletin, ferulic acid, and salicylic acid Glycyrrhiza echinata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.170 48700
-
 x * 48700, about, DNA sequence calculation Glycyrrhiza echinata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.170 additional information Glycyrrhiza echinata the enzyme might play a role in defense against external stresses such as UV radiation and pathogen attack ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.170 Glycyrrhiza echinata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.170 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography Glycyrrhiza echinata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.170 cell suspension culture transient induction by cell suspension culture treatment with yeast extract Glycyrrhiza echinata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.170 additional information the enzyme might play a role in defense against external stresses such as UV radiation and pathogen attack Glycyrrhiza echinata ?
-
 ?
2.4.1.170 additional information substrate specificity, the flavonoids kaempferol, apigenin, and quercetin are poor substrates Glycyrrhiza echinata ?
-
 ?
2.4.1.170 UDP-glucose + baicalein very low activity Glycyrrhiza echinata UDP + baicalein 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + daidzein
-
 Glycyrrhiza echinata UDP + daidzein 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + ferulic acid low activity Glycyrrhiza echinata UDP + 1-O-feruloyl-beta-D-glucose
-
 ?
2.4.1.170 UDP-glucose + formononetin best substrate Glycyrrhiza echinata UDP + formononetin 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + naringenin low activity Glycyrrhiza echinata UDP + naringenin 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + salicylic acid very low activity Glycyrrhiza echinata UDP + salicylic acid 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + scopoletin low activity Glycyrrhiza echinata UDP + scopoletin 7-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.170 ? x * 48700, about, DNA sequence calculation Glycyrrhiza echinata

Synonyms

EC Number Synonyms Comment Organism
2.4.1.170 More the enzyme belongs to the family of UDP-glycosyltransferases Glycyrrhiza echinata
2.4.1.170 UDP-glucose: formononetin 7-O-glucosyltransferase
-
 Glycyrrhiza echinata
2.4.1.170 UGT73F1
-
 Glycyrrhiza echinata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.170 30
-
 assay at Glycyrrhiza echinata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.170 8
-
 assay at Glycyrrhiza echinata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.170 Glycyrrhiza echinata sequence calculation
-
 5.6