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Literature summary extracted from

  • Chu, X.; Li, D.
    Expression, purification, and characterization of His20 mutants of rat mevalonate kinase (2003), Protein Expr. Purif., 32, 75-82.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 native and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.36 H20K expressed in inclusion bodies that can be solubilized in 8 M urea, refolding to a soluble protein was unsuccessful indicating irreversible structural changes Rattus norvegicus
2.7.1.36 H20L no significant changes in secondary structure, increased Km for both substrates Rattus norvegicus
2.7.1.36 H20Y no significant changes in secondary structure, increased Km for both substrates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 farnesyl diphosphate competitive with ATP Rattus norvegicus
2.7.1.36 geranyl diphosphate competitive with ATP Rattus norvegicus
2.7.1.36 geranylgeranyl diphosphate competitive with ATP Rattus norvegicus
2.7.1.36 prenyl phosphates competitive with ATP Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.035
-
(R)-mevalonate native enzyme, pH 7.5, 25°C Rattus norvegicus
2.7.1.36 0.616
-
(R)-mevalonate H20L mutant enzyme, pH 7.5, 25°C Rattus norvegicus
2.7.1.36 0.953
-
ATP native enzyme, pH 7.5, 25°C Rattus norvegicus
2.7.1.36 1.81
-
ATP H20L mutant enzyme, pH 7.5, 25°C Rattus norvegicus
2.7.1.36 2.16
-
(R)-mevalonate H20Y mutant enzyme, pH 7.5, 25°C Rattus norvegicus
2.7.1.36 2.7
-
ATP H20Y mutant enzyme enzyme, pH 7.5, 25°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.36 cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 42000
-
x * 42000, SDS-PAGE, His-tag protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Rattus norvegicus involved in cholesterol, steroid hormones und terpenoids synthesis ADP + (R)-5-phosphomevalonate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant proteins using His-tag Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 32.7
-
purified His-tag protein, pH 7.5, 25°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.36 4°C, 5% glycerol, 5 mM 2-mercaptoethanol, stable for more than 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
?
2.7.1.36 ATP + (R)-mevalonate involved in cholesterol, steroid hormones und terpenoids synthesis Rattus norvegicus ADP + (R)-5-phosphomevalonate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 ? x * 42000, SDS-PAGE, His-tag protein Rattus norvegicus