EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.36 | native and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.36 | H20K | expressed in inclusion bodies that can be solubilized in 8 M urea, refolding to a soluble protein was unsuccessful indicating irreversible structural changes | Rattus norvegicus |
2.7.1.36 | H20L | no significant changes in secondary structure, increased Km for both substrates | Rattus norvegicus |
2.7.1.36 | H20Y | no significant changes in secondary structure, increased Km for both substrates | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.36 | farnesyl diphosphate | competitive with ATP | Rattus norvegicus | |
2.7.1.36 | geranyl diphosphate | competitive with ATP | Rattus norvegicus | |
2.7.1.36 | geranylgeranyl diphosphate | competitive with ATP | Rattus norvegicus | |
2.7.1.36 | prenyl phosphates | competitive with ATP | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.36 | 0.035 | - |
(R)-mevalonate | native enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.7.1.36 | 0.616 | - |
(R)-mevalonate | H20L mutant enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.7.1.36 | 0.953 | - |
ATP | native enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.7.1.36 | 1.81 | - |
ATP | H20L mutant enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.7.1.36 | 2.16 | - |
(R)-mevalonate | H20Y mutant enzyme, pH 7.5, 25°C | Rattus norvegicus | |
2.7.1.36 | 2.7 | - |
ATP | H20Y mutant enzyme enzyme, pH 7.5, 25°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.36 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.36 | 42000 | - |
x * 42000, SDS-PAGE, His-tag protein | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.36 | ATP + (R)-mevalonate | Rattus norvegicus | involved in cholesterol, steroid hormones und terpenoids synthesis | ADP + (R)-5-phosphomevalonate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.36 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.36 | recombinant proteins using His-tag | Rattus norvegicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.36 | 32.7 | - |
purified His-tag protein, pH 7.5, 25°C | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.36 | 4°C, 5% glycerol, 5 mM 2-mercaptoethanol, stable for more than 1 month | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.36 | ATP + (R)-mevalonate | - |
Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
2.7.1.36 | ATP + (R)-mevalonate | involved in cholesterol, steroid hormones und terpenoids synthesis | Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.36 | ? | x * 42000, SDS-PAGE, His-tag protein | Rattus norvegicus |