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Literature summary extracted from

  • Ohta, S.; Ishida, Y.; Usami, S.
    Expression of cold-tolerant pyruvate, orthophosphate dikinase cDNA, and heterotetramer formation in transgenic maize plants (2004), Transgenic Res., 13, 475-485.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.9.1 agriculture modification of the cold sensitivity of a C4 photosynthetic enzyme by employing cereal transformation technology. A significant improvement in the cold stability of PPDK can be achieved when a suffcient quantity of cold-tolerant subunits is expressed in transgenic maize leaves Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 high levels of expression in Zea mays by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK Flaveria bidentis
2.7.9.1 introduction of a cold-tolerant PPDK cDNA isolated from Flaveria brownii into maize by Agrobacterium-mediated transformation. Higher levels of expression by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK Flaveria brownii
2.7.9.1 to improve the cold stability of the enzyme, a cold-tolerant PPDK cDNA isolated from Flaveria brownii is introduced into maize by Agrobacterium-mediated transformation. Higher levels of expression are ontained by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK. A significant improvement in the cold stability of PPDK can be achieved when a suffcient quantity of cold-tolerant subunits is expressed in transgenic maize leaves Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate Flaveria bidentis
-
 AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate Flaveria brownii
-
 AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate Zea mays loses activity below about 12°C by dissociation of the tetramer, considered as one possible cause of the reduction of the photosynthetic rate of maize at low temperatures AMP + phosphoenolpyruvate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Flaveria bidentis
-
-
-
2.7.9.1 Flaveria brownii
-
-
-
2.7.9.1 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Zea mays AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate
-
 Flaveria bidentis AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate
-
 Flaveria brownii AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate loses activity below about 12°C by dissociation of the tetramer, considered as one possible cause of the reduction of the photosynthetic rate of maize at low temperatures Zea mays AMP + phosphoenolpyruvate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 PPDK
-
 Zea mays
2.7.9.1 PPDK
-
 Flaveria bidentis
2.7.9.1 PPDK
-
 Flaveria brownii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.9.1 12
-
 loses activity below at about 12°C by dissociation of the tetramer, considered as one possible cause of the reduction of the photosynthetic rate of maize at low temperatures Zea mays