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Literature summary extracted from

  • Chen, Z.W.; Jiang, C.Y.; She, Q.; Liu, S.J.; Zhou, P.J.
    Key role of cysteine residues in catalysis and subcellular localization of sulfur oxygenase-reductase of Acidianus tengchongensis (2005), Appl. Environ. Microbiol., 71, 621-628.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.55 dithiothreitol 0.05 mM, 70°C, 109.3% activity Acidianus tengchongensis
1.13.11.55 dithiothreitol 0.05 mM, 9.3% increase of activity Acidianus tengchongensis
1.13.11.55 EDTA 10 mM, 70°C, 108.8% activity Acidianus tengchongensis
1.13.11.55 EDTA 0.05 mM, 8.8% increase of activity Acidianus tengchongensis
1.13.11.55 glutathione 0.05 mM, 70°C, 110% activity Acidianus tengchongensis
1.13.11.55 GSH 0.05 mM, 10.8% increase of activity Acidianus tengchongensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.55 expression in Escherichia coli HB101 Acidianus tengchongensis
1.13.11.55 overexpression of wild-type and mutant enzymes in Escherichia coli Acidianus tengchongensis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.55 C101S mutant with reduced activity Acidianus tengchongensis
1.13.11.55 C101S 98.4% loss of activity Acidianus tengchongensis
1.13.11.55 C104S mutant with reduced activity Acidianus tengchongensis
1.13.11.55 C104S 99.2% loss of activity Acidianus tengchongensis
1.13.11.55 C31A complete loss of activity Acidianus tengchongensis
1.13.11.55 C31A mutant with reduced activity Acidianus tengchongensis
1.13.11.55 C31S complete loss of activity Acidianus tengchongensis
1.13.11.55 C31S mutant with reduced activity Acidianus tengchongensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.55 Co2+ 1 mM,89.2% inhibition Acidianus tengchongensis
1.13.11.55 CoCl2 1 mM, 70°C, 10.8% activity Acidianus tengchongensis
1.13.11.55 Cu2+ 1 mM, 99.3% inhibition Acidianus tengchongensis
1.13.11.55 CuCl2 1 mM, 70°C, 0.7% activity Acidianus tengchongensis
1.13.11.55 Mg2+ 1 mM, 22.8% inhibition Acidianus tengchongensis
1.13.11.55 MgCl2 1 mM, 70°C, 77.2% activity Acidianus tengchongensis
1.13.11.55 Mn2+ 1 mM, 65.5% inhibition Acidianus tengchongensis
1.13.11.55 MnCl2 1 mM, 70°C, 34.5% activity Acidianus tengchongensis
1.13.11.55 N-ethylmaleimide 0.1-1 mM, 70°C, 0.02-0.03% activity Acidianus tengchongensis
1.13.11.55 NEM 0.1 mM, complete Acidianus tengchongensis
1.13.11.55 Ni2+ 1 mM, 87.5% inhibition Acidianus tengchongensis
1.13.11.55 NiCl2 1 mM, 70°C, 12.5% activity Acidianus tengchongensis
1.13.11.55 Zn2+ 1 mM, 70°C, 27% activity; 1 mM, 73% inhibition Acidianus tengchongensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.55 cytoplasm
-
 Acidianus tengchongensis 5737
-
1.13.11.55 cytoplasm also associated with the cytoplasmic membrane Acidianus tengchongensis 5737
-
1.13.11.55 cytoplasmic membrane membrane-associated enzyme activity is colocalized with the activities of sulfite:acceptor oxidoreductase and thiosulfate:acceptor oxidoreductase to catalyze sulfur oxidation Acidianus tengchongensis
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.55 Acidianus tengchongensis
-
-
-
1.13.11.55 Acidianus tengchongensis S5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.55
-
 Acidianus tengchongensis
1.13.11.55 Superdex 200 gel-filtration Acidianus tengchongensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.55 0.04
-
 mutant C104S, formation of sulfite plus thiosulfate, 70°C Acidianus tengchongensis
1.13.11.55 0.074
-
 wild type in cellular lysate, formation of sulfite plus thiosulfate, 70°C Acidianus tengchongensis
1.13.11.55 0.08
-
 mutant C101S, formation of sulfite plus thiosulfate, 70°C Acidianus tengchongensis
1.13.11.55 0.1078
-
 wild type in supernatant, formation of sulfite plus thiosulfate, 70°C Acidianus tengchongensis
1.13.11.55 0.476
-
 wild type in pellet, formation of sulfite plus thiosulfate, 70°C Acidianus tengchongensis
1.13.11.55 4.85
-
 wild type, formation of sulfite plus thiosulfate, 70°C Acidianus tengchongensis

Storage Stability

EC Number Storage Stability Organism
1.13.11.55 -20°C Acidianus tengchongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.55 S + O2 + H2O
-
 Acidianus tengchongensis HSO3- + H2S + H+
-
 ?
1.13.11.55 S + O2 + H2O
-
 Acidianus tengchongensis S5 HSO3- + H2S + H+
-
 ?