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Literature summary extracted from

  • Rosano, C.; Zuccotti, S.; Cobucci-Ponzano, B.; Mazzone, M.; Rossi, M.; Moracci, M.; Petoukhov, M.V.; Svergun, D.I.; Bolognesi, M.
    Structural characterization of the nonameric assembly of an Archaeal alpha-L-fucosidase by synchroton small angle X-ray scattering (2004), Biochem. Biophys. Res. Commun., 320, 176-182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 expression in Escherichia coli Saccharolobus solfataricus
3.2.1.127 expression in Escherichia coli BL21(DE3) Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.51 recombinant enzyme, vapor diffusion method Saccharolobus solfataricus
3.2.1.127
-
 Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.127 lysosome
-
 Saccharolobus solfataricus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 57400
-
 9 * 57400, calculated from sequence Saccharolobus solfataricus
3.2.1.51 473000
-
 gel filtration Saccharolobus solfataricus
3.2.1.127 57000
-
 9 * 57000, SDS-PAGE, crystal structure Saccharolobus solfataricus
3.2.1.127 57390
-
 monomer, calculated from amino acid sequence Saccharolobus solfataricus
3.2.1.127 508000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Saccharolobus solfataricus
-
-
-
3.2.1.127 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 recombinant Saccharolobus solfataricus
3.2.1.127
-
 Saccharolobus solfataricus

Subunits

EC Number Subunits Comment Organism
3.2.1.51 nonamer 9 * 57400, calculated from sequence Saccharolobus solfataricus
3.2.1.127 nonamer 9 * 57000, SDS-PAGE, crystal structure Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.127 alpha-L-fucosidase
-
 Saccharolobus solfataricus
3.2.1.127 Ss-alpha-fuc
-
 Saccharolobus solfataricus