Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fromant, M.; Scvhmitt, E.; Mechulam, Y.; Lazennec, C.; Plateau, P.; Blanquet, S.
    Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase (2005), Biochemistry, 44, 4292-4301.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.29 hanging drop vapour diffusion method, 1.8 A resolution Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.29 D86A kcat/Km for diacetyl-lysyl-tRNALys is 0.22% of wild-type value Saccharolobus solfataricus
3.1.1.29 D86A/K18A kcat/Km for diacetyl-lysyl-tRNALys is 0.17% of wild-type value Saccharolobus solfataricus
3.1.1.29 H25A kcat/Km for diacetyl-lysyl-tRNALys is 13% of wild-type value Saccharolobus solfataricus
3.1.1.29 K118A kcat/Km for diacetyl-lysyl-tRNALys is 87% of wild-type value Saccharolobus solfataricus
3.1.1.29 K18A kcat/Km for diacetyl-lysyl-tRNALys is 0.2% of wild-type value Saccharolobus solfataricus
3.1.1.29 K56A kcat/Km for diacetyl-lysyl-tRNALys is 12% of wild-type value Saccharolobus solfataricus
3.1.1.29 Q22A kcat/Km for diacetyl-lysyl-tRNALys is 20% of wild-type value Saccharolobus solfataricus
3.1.1.29 Q54A kcat/Km for diacetyl-lysyl-tRNALys is 47% of wild-type value Saccharolobus solfataricus
3.1.1.29 T90A kcat/Km for diacetyl-lysyl-tRNALys is 1.4% of wild-type value Saccharolobus solfataricus
3.1.1.29 T98A kcat/Km for diacetyl-lysyl-tRNALys is 34% of wild-type value Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.29 0.9
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T98A Saccharolobus solfataricus
3.1.1.29 1.6
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q22A Saccharolobus solfataricus
3.1.1.29 2.9
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q54A Saccharolobus solfataricus
3.1.1.29 3.2
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K56A Saccharolobus solfataricus
3.1.1.29 4.4
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme H25A Saccharolobus solfataricus
3.1.1.29 5.3
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K18A Saccharolobus solfataricus
3.1.1.29 5.4
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A Saccharolobus solfataricus
3.1.1.29 6.7
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A/K18A Saccharolobus solfataricus
3.1.1.29 7.2
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T90A Saccharolobus solfataricus
3.1.1.29 9.1
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.29 12.8
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K118A Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 wild-type and mutant enzymes Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 diacetyl-lysyl-tRNALys
-
 Saccharolobus solfataricus diacetyl-Lys + tRNALys
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 YbaK
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.29 0.002
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A/K18A Saccharolobus solfataricus
3.1.1.29 0.002
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K18A Saccharolobus solfataricus
3.1.1.29 0.0025
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A Saccharolobus solfataricus
3.1.1.29 0.02
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T90A Saccharolobus solfataricus
3.1.1.29 0.06
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q22A Saccharolobus solfataricus
3.1.1.29 0.06
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T98A Saccharolobus solfataricus
3.1.1.29 0.07
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K56A Saccharolobus solfataricus
3.1.1.29 0.1
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme H25A Saccharolobus solfataricus
3.1.1.29 0.27
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q54A Saccharolobus solfataricus
3.1.1.29 1.8
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.29 2.2
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K118A Saccharolobus solfataricus