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Literature summary extracted from

  • Park, S.; Cha, H.; Kang, H.; Shim, J.; Woo, E.; Kim, J.; Park, K.
    Mutagenesis of Ala290, which modulates substrate subsite affinity at the catalytic interface of dimeric ThMA (2005), Biochim. Biophys. Acta, 1751, 170-177.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.133 A290I mutant enzyme A290I produces mostly maltose from maltotetraose, while wild-type enzyme produces glucose as well as maltose. kcat/KM of mutant enzyme 290I for maltose is 48times less than that of wild-type enzyme. kcat/Km for maltotriose is 18.5fold lower than wild-type enzyme. kcat/Km for maltotetraose is 2.9fold lower than wild-type enzyme. kcat/Km for maltopentaose is 2.5fold lower than wild-type enzyme. kcat/Km for maltohexaose is 1.9fold lower than wild-type enzyme. kcat/Km for maltoheptaose is 4.7fold lower than wild-type enzyme Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.133 1.13
-
 maltotriose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 2.52
-
 maltotriose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 2.95
-
 maltotetraose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 3.38
-
 maltotetraose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 3.5
-
 maltopentaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 3.97
-
 maltopentaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 7.16
-
 maltohexaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 7.53
-
 maltohexaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 8.18
-
 maltoheptaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 9.21
-
 maltoheptaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 63.43
-
 maltose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 427
-
 maltose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Thermus sp.
-
 recombinant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 wild-type and mutant enzyme A290I Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 maltoheptaose + H2O
-
 Thermus sp. maltose + D-glucose + ? mutant enzyme A290I produces mostly maltose, while wild-type enzyme produces glucose (32.8%) as well as maltose ?
3.2.1.133 maltohexaose + H2O
-
 Thermus sp. ?
-
 ?
3.2.1.133 maltopentaose + H2O
-
 Thermus sp. ?
-
 ?
3.2.1.133 maltose + H2O
-
 Thermus sp. 2 D-glucose
-
 ?
3.2.1.133 maltotetraose + H2O
-
 Thermus sp. maltose + 2 D-glucose mutant enzyme A290I produces mostly maltose, while wild-type enzyme produces glucose (24.8%) as well as maltose ?
3.2.1.133 maltotriose + H2O
-
 Thermus sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 ThMA
-
 Thermus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.133 0.037 0.23 maltotriose 50°C, pH 6.0, mutant enzyme A29I Thermus sp.
3.2.1.133 0.04
-
 maltose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 0.29
-
 maltose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 2
-
 maltoheptaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 9.2
-
 maltohexaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 20 50 maltohexaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 29.8
-
 maltotetraose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 42.5
-
 maltoheptaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 69.01
-
 maltopentaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 72.08
-
 maltoheptaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 79.07
-
 maltotriose 50°C, pH 6.0, mutant enzyme A29I Thermus sp.
3.2.1.133 88.45
-
 maltohexaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 160.3
-
 maltohexaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 173.5
-
 maltotetraose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.2.1.133 197
-
 maltopentaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 384.1
-
 maltoheptaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 574.1
-
 maltotetraose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.2.1.133 652.5
-
 maltotriose 50°C, pH 6.0, wild-type enzyme Thermus sp.