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Literature summary extracted from

  • Kaulpiboon, J.; Pongsawasdi, P.
    Purification and characterization of cyclodextrinase from Paenibacillus sp. A11 (2005), Enzyme Microb. Technol., 36, 168-175.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide 5 mM, 37% inhibition Paenibacillus sp.
3.2.1.54 acarbose 5 mM, 91% inhibition Paenibacillus sp.
3.2.1.54 AgNO3 10 mM, complete inhibition Paenibacillus sp.
3.2.1.54 CuSO4 10 mM, complete inhibition Paenibacillus sp.
3.2.1.54 diethylpyrocarbonate 1 mM, complete inhibition Paenibacillus sp.
3.2.1.54 FeSO4 10 mM, 68% inhibition Paenibacillus sp.
3.2.1.54 HgCl2 10 mM, complete inhibition Paenibacillus sp.
3.2.1.54 iodoacetamide 5 mM, 49% inhibition Paenibacillus sp.
3.2.1.54 MgCl2 10 mM, 35% inhibition Paenibacillus sp.
3.2.1.54 MnCl2 10 mM, 33% inhibition Paenibacillus sp.
3.2.1.54 N-bromosuccinimide 89% inhibition at 0.1 mM, complete inhibition at 1 mM Paenibacillus sp.
3.2.1.54 Phenylglyoxal 5 mM, 60% inhibition Paenibacillus sp.
3.2.1.54 ZnCl2 10 mM, 78% inhibition Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 2.47
-
 beta-cyclodextrin pH 7.0, 40°C Paenibacillus sp.
3.2.1.54 3.1
-
 gamma-cyclodextrin pH 7.0, 40°C Paenibacillus sp.
3.2.1.54 5.66
-
 alpha-cyclodextrin pH 7.0, 40°C Paenibacillus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.54 intracellular
-
 Paenibacillus sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 80000
-
 gel filtration Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.54 additional information Paenibacillus sp. inducible enzyme ?
-
 ?
3.2.1.54 additional information Paenibacillus sp. A11 inducible enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Paenibacillus sp.
-
-
-
3.2.1.54 Paenibacillus sp. A11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54
-
 Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 additional information
-
-
 Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O 53% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 dextrin + H2O 29% of the activity with beta-cyclodextrin Paenibacillus sp. ?
-
 ?
3.2.1.54 dextrin + H2O 29% of the activity with beta-cyclodextrin Paenibacillus sp. A11 ?
-
 ?
3.2.1.54 G1-alpha-cyclodextrin + H2O 24% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 G1-beta-cyclodextrin + H2O 32% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 G2-alpha-cyclodextrin + H2O 19% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 G2-beta-cyclodextrin + H2O 30% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 gamma-cyclodextrin + H2O 59% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose main products ?
3.2.1.54 maltoheptaose + H2O 41% of the activity with beta-cyclodextrin Paenibacillus sp. ?
-
 ?
3.2.1.54 maltohexaose + H2O 33% of the activity with beta-cyclodextrin Paenibacillus sp. ?
-
 ?
3.2.1.54 maltohexaose + H2O 33% of the activity with beta-cyclodextrin Paenibacillus sp. A11 ?
-
 ?
3.2.1.54 maltopentaose + H2O 18% of the activity with beta-cyclodextrin Paenibacillus sp. ?
-
 ?
3.2.1.54 maltopentaose + H2O 18% of the activity with beta-cyclodextrin Paenibacillus sp. A11 ?
-
 ?
3.2.1.54 maltotetraose + H2O 12% of the activity with beta-cyclodextrin Paenibacillus sp. ?
-
 ?
3.2.1.54 maltotetraose + H2O 12% of the activity with beta-cyclodextrin Paenibacillus sp. A11 ?
-
 ?
3.2.1.54 maltotriose + H2O 11% of the activity with beta-cyclodextrin Paenibacillus sp. ?
-
 ?
3.2.1.54 additional information inducible enzyme Paenibacillus sp. ?
-
 ?
3.2.1.54 additional information inducible enzyme Paenibacillus sp. A11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 monomer 1 * 80000, SDS-PAGE Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 40
-
-
 Paenibacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 20 70 20°C: about 65% of maximal activity, 70°C: about 40% of maximal activity Paenibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.54 800
-
 alpha-cyclodextrin pH 7.0, 40°C Paenibacillus sp.
3.2.1.54 1155
-
 gamma-cyclodextrin pH 7.0, 40°C Paenibacillus sp.
3.2.1.54 2044
-
 beta-cyclodextrin pH 7.0, 40°C Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 7
-
-
 Paenibacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 5 8 about 50% of maximal activity at pH 5.0 and 8.0 Paenibacillus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.54 Paenibacillus sp. isoeletric focusing, pH-range 3-9
-
 5.4