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Literature summary extracted from

  • Krawetz, J.E.; Boston, R.S.
    Substrate specificity of a maize ribosome-inactivating protein differs across diverse taxa (2000), Eur. J. Biochem., 267, 1966-1974.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.22 additional information construction of an active deletion mutant MOD1 of recombinant proRIP1 with reduced activity compared to recombinant RIP1 after activation of both by papain Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.22 Mg2+ activates Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.22 large rRNA + H2O Zea mays inhibition of translation and protein synthesis, cytotoxic ?
-
 ?
3.2.2.22 additional information Zea mays the isozyme RIP1 has defense-related function in maize plants ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Zea mays P25891
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.2.22 proteolytic modification isozyme RIP1 is synthesized as inactive precursor protein B-32 or proRIP, which is activated by proteolytic cleavage into two polypeptide chains forming the dimeric enzyme, the enzyme can be activated by papain Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.22 endosperm
-
 Zea mays
-
3.2.2.22 kernel
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.22 additional information
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 large rRNA + H2O inhibition of translation and protein synthesis, cytotoxic Zea mays ?
-
 ?
3.2.2.22 large rRNA + H2O substrate are intact rabbit ribosomes, best substrate, or ribosomes from Aspergillus flavus, low activity, or Zea mays, the latter are poor substrates, depurination of specific sites in rRNA, formation of RNA fragments Zea mays large rRNA fragments, partially deadenylated fragment size overview, product determination using aniline coupling ?
3.2.2.22 additional information the isozyme RIP1 has defense-related function in maize plants Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.22 B-32
-
 Zea mays
3.2.2.22 ribosome-inactivating protein 3
-
 Zea mays
3.2.2.22 RIP1
-
 Zea mays
3.2.2.22 RNA-specific N-glycosidase
-
 Zea mays
3.2.2.22 rRNA N-glycosidase
-
 Zea mays
3.2.2.22 type 3 RIP
-
 Zea mays
3.2.2.22 type III ribosome-inactivating protein
-
 Zea mays
3.2.2.22 type III RIP
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.22 25
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.22 7.5
-
 assay at Zea mays