Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Su, C.; Wang, D.; Yao, L.; Yu, Z.
    Purification, characterization, and gene cloning of a chitosanase from Bacillus species strain S65 (2006), J. Agric. Food Chem., 54, 4208-4214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 CaCl2 10 mM, 48% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 CoCl2 10 mM, 33% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 CuCl2 10 mM, 69% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 FeCl2 1 mM, 80.7% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 FeCl3 1 mM, 84.8% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 ZnCl2 10 mM, 42.8% inhibition Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 CoCl2 5 mM, 15% enhancement of activity Bacillus sp. (in: Bacteria)
3.2.1.132 CuCl2 1 mM, 19% enhancement of activity Bacillus sp. (in: Bacteria)
3.2.1.132 MnCl2 1 mM, 41.2% enhancement of activity Bacillus sp. (in: Bacteria)
3.2.1.132 ZnCl2 1 mM, 24.3% enhancement of activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 45000
-
 x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.132 Bacillus sp. (in: Bacteria) S65
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 culture medium enzyme is constitutively produced Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 93.6
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 carboxymethyl cellulose + H2O 5.3% of the activity with soluble chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 carboxymethyl cellulose + H2O 5.3% of the activity with soluble chitosan Bacillus sp. (in: Bacteria) S65 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus sp. (in: Bacteria) S65 ?
-
 ?
3.2.1.132 additional information the enzyme cannot hydrolyze colloidal chitin and crystalline cellulose Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 additional information the enzyme cannot hydrolyze colloidal chitin and crystalline cellulose Bacillus sp. (in: Bacteria) S65 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 65
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 55 70 55°C: about 85% of maximal activity, 70°C: about 90% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 40
-
 pH 6, 1 h, stable below Bacillus sp. (in: Bacteria)
3.2.1.132 50
-
 pH 6, 1 h, about 50% loss of activity Bacillus sp. (in: Bacteria)
3.2.1.132 65
-
 pH 6, 10 min, about 90% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 5.5 6.5 28°C, 60 min, stable Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Bacillus sp. (in: Bacteria)
-
-
 6