Any feedback?
Literature summary extracted from
- Aristolochene synthase: mechanistic analysis of active site residues by site-directed mutagenesis (2004), J. Am. Chem. Soc., 126, 7212-7221.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.3.9 | D115E | kcat/KM is 12fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 75:6:119 | Penicillium roqueforti |
| 4.2.3.9 | D115N | inactive mutant enzyme | Penicillium roqueforti |
| 4.2.3.9 | D116E | kcat/KM is 60fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 62:3:35 | Penicillium roqueforti |
| 4.2.3.9 | D116N | kcat/KM is 48fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 63:2:35 | Penicillium roqueforti |
| 4.2.3.9 | E119D | kcat/KM is 2.4fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 94:2:4 | Penicillium roqueforti |
| 4.2.3.9 | E119Q | kcat/KM is 2.3fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 84:2:14 | Penicillium roqueforti |
| 4.2.3.9 | E227D | kcat/KM is 1182fold higher than wild-type value, in contrast to wild-type enzyme that exclusively produces aristolochene from trans,trans-farnesyl diphosphate, the mutant enzyme produces 26% aristolochene and 74% germacrene A | Aspergillus terreus |
| 4.2.3.9 | E227Q | inactive mutant enzyme | Aspergillus terreus |
| 4.2.3.9 | E252D | kcat/KM is 111fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 19:0:81 | Penicillium roqueforti |
| 4.2.3.9 | E252Q | mutant enzyme produces only (-)-germacrene A | Penicillium roqueforti |
| 4.2.3.9 | N219D | kcat/KM is 6667fold higher than wild-type value, in contrast to wild-type enzyme that exclusively produces aristolochene from trans,trans-farnesyl diphosphate, the mutant enzyme produces 44% aristolochene and 56% germacrene A | Aspergillus terreus |
| 4.2.3.9 | N244D | kcat/KM is 1978fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 19:0:81 | Penicillium roqueforti |
| 4.2.3.9 | N244L | inactive mutant enzyme | Penicillium roqueforti |
| 4.2.3.9 | S248A | kcat/KM is 300fold lower than wild-type value. Wild-type enzyme produces (+)-aristolochene, (-)-valencene and (S)-(-)-germacrene A in the ratio 93:2:4, the ratio of the mutant enzyme is 21:0:79 | Penicillium roqueforti |
| 4.2.3.9 | S248A/E252D | inactive mutant enzyme | Penicillium roqueforti |
| 4.2.3.9 | Y92F | 100fold reduction in kcat, 50fold decrease in KM, resulting in 2fold decrease in kcat/Km. The mutant enzyme produces (+)-aristolochene as 81% of the product, 7% (-)-valencene and 12% (S)-(-)-germacrene A | Penicillium roqueforti |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.9 | 0.000012 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme Y92F | Penicillium roqueforti |  |
| 4.2.3.9 | 0.00015 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E119Q | Penicillium roqueforti | |
| 4.2.3.9 | 0.00032 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E119D | Penicillium roqueforti | |
| 4.2.3.9 | 0.0006 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, wild-type enzyme | Penicillium roqueforti | |
| 4.2.3.9 | 0.0011 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme D116E | Penicillium roqueforti | |
| 4.2.3.9 | 0.0013 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, wild-type enzyme | Aspergillus terreus | |
| 4.2.3.9 | 0.0014 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E227D | Aspergillus terreus | |
| 4.2.3.9 | 0.0015 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme D116N | Penicillium roqueforti | |
| 4.2.3.9 | 0.0027 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme D115E | Penicillium roqueforti | |
| 4.2.3.9 | 0.0033 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme N244D | Penicillium roqueforti | |
| 4.2.3.9 | 0.0034 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E252D | Penicillium roqueforti | |
| 4.2.3.9 | 0.0039 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme N219D | Aspergillus terreus | |
| 4.2.3.9 | 0.0055 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme S248A | Penicillium roqueforti | |
| 4.2.3.9 | 0.0101 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E252Q | Penicillium roqueforti | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.9 | Aspergillus terreus | - |
- |
- |
| 4.2.3.9 | Penicillium roqueforti | Q03471 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.9 | trans,trans-farnesyl diphosphate | - |
Penicillium roqueforti | (+)-aristolochene + diphosphate | (+)-aristolochene + minor amounts of (S)-(-)-germacrene A and (-)-valencene in a 94:4:2 ratio | ? | |
| 4.2.3.9 | trans,trans-farnesyl diphosphate | - |
Aspergillus terreus | (+)-aristolochene + diphosphate | (+)-aristolochene is the only product | ? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.9 | 0.000076 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme N219D | Aspergillus terreus | |
| 4.2.3.9 | 0.00012 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme N244D | Penicillium roqueforti | |
| 4.2.3.9 | 0.00022 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E252Q | Penicillium roqueforti | |
| 4.2.3.9 | 0.0004 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme Y92F | Penicillium roqueforti | |
| 4.2.3.9 | 0.0013 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme D116E | Penicillium roqueforti | |
| 4.2.3.9 | 0.0013 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme S248A | Penicillium roqueforti | |
| 4.2.3.9 | 0.0016 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E227D | Aspergillus terreus | |
| 4.2.3.9 | 0.0022 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme D116N | Penicillium roqueforti | |
| 4.2.3.9 | 0.0022 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E252D | Penicillium roqueforti | |
| 4.2.3.9 | 0.0048 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E119Q | Penicillium roqueforti | |
| 4.2.3.9 | 0.0097 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme E119D | Penicillium roqueforti | |
| 4.2.3.9 | 0.0162 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, mutant enzyme D115E | Penicillium roqueforti | |
| 4.2.3.9 | 0.0173 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, wild-type enzyme | Aspergillus terreus | |
| 4.2.3.9 | 0.043 | - |
trans,trans-farnesyl diphosphate | pH 8.0, 30°C, wild-type enzyme | Penicillium roqueforti | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
