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Literature summary extracted from

  • Nardini, M.; Lang, D.A.; Liebeton, K.; Jaeger, K.E.; Dijkstra, B.W.
    Crystal structure of Pseudomonas aeruginosa lipase in the open conformation. The prototype for family 1.1 of bacterial lipases (2000), J. Biol. Chem., 275, 31219-31225.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.3 purified recombinant enzyme in open conformation through complexion by inhibitor Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-(4-nitrophenyl) octylphosphonate, sitting drop vapour diffusion method, room temperature, 5 mg/ml protein in10 mM Tris-HCl, pH 8.0, 1% beta-octylpyranoside, equilibration against precipitation solution containing 25% 2-methyl-2,4-pentanediol, 20 mM CaCl2, 100 mM citrate, pH 5.6, several months, X-ray structure determination and analysis at 2.54 A resolution, structure scheme and modelling Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-(4-nitrophenyl) octylphosphonate binding site structure, binding arrests the enzyme in the open conformation Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ stabilizes the loop containing the catalytic His251 residue, binding site structure Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa P26876
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate the catalytic triad is formed by Ser82, Asp229, and His251, substrates bind first to Ser82, position of the oxyanion hole and the three pockets accomodating the sn-1, sn-2, and sn-3 fatty acid chains, reaction mechanism, tetrahedral intermediate during acylation step Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 triacylglycerol + H2O
-
 Pseudomonas aeruginosa diacylglycerol + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 More the enzyme structure contains an unusual alpha/beta hydrolase fold lacking two beta-strands and one helix Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Pseudomonas aeruginosa
3.1.1.3 More the enzyme belongs to the family 1.1 of bacterial lipases Pseudomonas aeruginosa