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Literature summary extracted from

  • Li, J.F.; Qu, L.H.; Li, N.
    Tyr152 plays a central role in the catalysis of 1-aminocyclopropane-1-carboxylate synthase (2005), J. Exp. Bot., 56, 2203-2210.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.14 overexpression of isoenzyme LeACS2 and five ACC synthase mutants (Y151F, Y151G, Y152F, Y152G and Y151F/Y152F) in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.14 Y151F activity is reduced by 27% Solanum lycopersicum
4.4.1.14 Y151F/Y152F activity is reduced by 99% Solanum lycopersicum
4.4.1.14 Y151G activity is reduced by 83% Solanum lycopersicum
4.4.1.14 Y152F activity is reduced by 98% Solanum lycopersicum
4.4.1.14 Y152G inactive mutant enzyme Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.14 0.0315
-
 S-adenosyl-L-methionine 30°C, pH 8.5, wild-type enzyme Solanum lycopersicum
4.4.1.14 0.0458
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F Solanum lycopersicum
4.4.1.14 0.168
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151G Solanum lycopersicum
4.4.1.14 0.409
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F/Y152F Solanum lycopersicum
4.4.1.14 0.514
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y152F Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.14 additional information Solanum lycopersicum key enzyme in the regulation of ethylene biosynthesis in higher plants ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.14 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.14 isoenzyme LeACS2 and five ACC synthase mutants (Y151F, Y151G, Y152F, Y152G and Y151F/Y152F) in Escherichia coli Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.14 additional information key enzyme in the regulation of ethylene biosynthesis in higher plants Solanum lycopersicum ?
-
 ?
4.4.1.14 S-adenosyl-L-methionine
-
 Solanum lycopersicum 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.14 LeACS2
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.14 0.18
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F/Y152F Solanum lycopersicum
4.4.1.14 0.39
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y152F Solanum lycopersicum
4.4.1.14 1.3
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151G Solanum lycopersicum
4.4.1.14 1.41
-
 S-adenosyl-L-methionine 30°C, pH 8.5, wild-type enzyme Solanum lycopersicum
4.4.1.14 1.5
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F Solanum lycopersicum