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Literature summary extracted from
- The endonuclease Hje catalyses rapid, multiple turnover resolution of Holliday junctions (2005), J. Mol. Biol., 350, 1-6.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | expression in Escherichia coli using vector pET19b-Hje with a native N-terminal | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.21.10 | 0.000066 | - |
DNA junction 1 | at 15 mM MgCl2, 65°C, pH 7 | Saccharolobus solfataricus | |
| 3.1.21.10 | 0.000073 | - |
DNA junction 1 | at 5 mM MgCl2, 65°C, pH 7 | Saccharolobus solfataricus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.10 | Mg2+ | initiation of reaction with 15 mM MgCl2 at 65°C | Saccharolobus solfataricus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.10 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | purified to homogeneity | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.10 | DNA junction 1 + H2O | DNA junction 1 substrate assembled from strands b50, h50,r50,x50, r55, b1-27, b28-50 | Saccharolobus solfataricus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | endonuclease Hje | - |
Saccharolobus solfataricus |
| 3.1.21.10 | Hje | - |
Saccharolobus solfataricus |
| 3.1.21.10 | Hje endonuclease | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.10 | 30 | - |
steady-state approximation at 0.30 min-1 under maximal velocity conditions at 15 mM MgCl2 | Saccharolobus solfataricus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.10 | 45 | 65 | no multiple turnover catalysis at lower temperatures measurable due to the sensitivity of the assay | Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.21.10 | 0.0065 | - |
DNA junction 1 | at 30°C, steady-state approximation under maximal velocity conditions at 15 mM MgCl2 | Saccharolobus solfataricus | |
| 3.1.21.10 | 0.05 | - |
DNA junction 1 | at 65°C, pH 7, converting 6% of the substrate into product | Saccharolobus solfataricus |
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