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Literature summary extracted from

  • Yamasaki, Y.; Fujimoto, M.; Kariya, J.; Konno, H.
    Purification and characterization of an alpha-glucosidase from germinating millet seeds (2005), Phytochemistry, 66, 851-857.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Hg2+ at least 80% inhibition at 5 mM Panicum miliaceum
3.2.1.20 Pb2+ at least 80% inhibition at 5 mM Panicum miliaceum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information the KM value decreases with the increase in molecular weight of substrates Panicum miliaceum
3.2.1.20 0.0013
-
 amylopectin pH 4.5, 37°C Panicum miliaceum
3.2.1.20 0.28
-
 amylose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 0.34
-
 kojibiose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 0.39
-
 maltoheptaose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 0.45
-
 maltohexaose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 0.86
-
 maltotetraose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 1.13
-
 maltotriose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 1.46
-
 maltose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 1.46
-
 nigerose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 3.9
-
 phenyl alpha-glucoside pH 4.5, 37°C Panicum miliaceum
3.2.1.20 9.76
-
 isomaltose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 3640
-
 soluble starch pH 4.5, 37°C Panicum miliaceum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 additional information the enzyme is not affected by 5 mM of Na+, K+, Ca2+, Co2+, Mn2+, Ni2+, Mg2+, Sr2+, or Al3+ Panicum miliaceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 80000
-
 gel filtration Panicum miliaceum
3.2.1.20 86000
-
 1 * 86000, SDS-PAGE Panicum miliaceum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 alpha-1,4-glucan + H2O Panicum miliaceum
-
 alpha-D-glucose
-
 ?
3.2.1.20 amylopectin + H2O Panicum miliaceum in starch, preferred substrate alpha-D-glucose
-
 ?
3.2.1.20 malto-oligosaccharides + H2O Panicum miliaceum
-
 alpha-D-glucose
-
 ?
3.2.1.20 maltoheptaose + 6 H2O Panicum miliaceum
-
 7 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O Panicum miliaceum
-
 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 additional information Panicum miliaceum the enzyme prefers partially degraded starch substrate from germinating millet seeds ?
-
 ?
3.2.1.20 nigerose + H2O Panicum miliaceum alpha-1,3-linkage, exists as abnormal linkage in starch 2 alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Panicum miliaceum
-
 alpha-glucosidase II in germinating seeds
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 native enzyme from germinating millet seeds to homogeneity by ammonium sulfate fractionation, cation exchange chromatography, gel filtration, hydrophobic interaction chromatography and preparative isoelectric focusing Panicum miliaceum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 seed germinating, alpha-glucosidase II Panicum miliaceum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
-
 Panicum miliaceum
3.2.1.20 76
-
 purified native enzyme Panicum miliaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 alpha-1,4-glucan + H2O
-
 Panicum miliaceum alpha-D-glucose
-
 ?
3.2.1.20 amylopectin + H2O preferred substrate Panicum miliaceum alpha-D-glucose
-
 ?
3.2.1.20 amylopectin + H2O in starch, preferred substrate Panicum miliaceum alpha-D-glucose
-
 ?
3.2.1.20 amylose + H2O amylose EX-1 Panicum miliaceum alpha-D-glucose
-
 ?
3.2.1.20 isomaltose + H2O very low activity Panicum miliaceum 2 alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Panicum miliaceum alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 malto-oligosaccharides + H2O
-
 Panicum miliaceum alpha-D-glucose
-
 ?
3.2.1.20 maltoheptaose + 6 H2O
-
 Panicum miliaceum 7 alpha-D-glucose
-
 ?
3.2.1.20 maltohexaose + 5 H2O
-
 Panicum miliaceum 6 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Panicum miliaceum alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + 3 H2O
-
 Panicum miliaceum 4 alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + 2 H2O
-
 Panicum miliaceum 3 alpha-D-glucose
-
 ?
3.2.1.20 additional information the enzyme prefers partially degraded starch substrate from germinating millet seeds Panicum miliaceum ?
-
 ?
3.2.1.20 additional information the enzyme hydrolyzes polymer substrate more rapidly than maltose, substrate specificity, activity with insoluble substrates, overview Panicum miliaceum ?
-
 ?
3.2.1.20 nigerose + H2O alpha-1,3-linkage, exists as abnormal linkage in starch Panicum miliaceum 2 alpha-D-glucose
-
 ?
3.2.1.20 phenyl alpha-glucoside + H2O low activity Panicum miliaceum alpha-D-glucose + phenol
-
 ?
3.2.1.20 soluble starch + H2O very low activity Panicum miliaceum alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 86000, SDS-PAGE Panicum miliaceum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase II
-
 Panicum miliaceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 50 60
-
 Panicum miliaceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 45
-
 purified native enzyme, stable up to Panicum miliaceum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 0.04 1.97 kojibiose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 3.8
-
 phenyl alpha-glucoside pH 4.5, 37°C Panicum miliaceum
3.2.1.20 5.75
-
 isomaltose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 9.44
-
 kojibiose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 24.2
-
 amylopectin pH 4.5, 37°C Panicum miliaceum
3.2.1.20 26.2
-
 amylose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 33.5
-
 maltose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 35.7
-
 nigerose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 36.1
-
 maltohexaose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 40.7
-
 maltotetraose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 43.9
-
 maltotriose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 47.5
-
 maltoheptaose pH 4.5, 37°C Panicum miliaceum
3.2.1.20 49.7
-
 soluble starch pH 4.5, 37°C Panicum miliaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5 5
-
 Panicum miliaceum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 2.5 8 purified native enzyme, stable Panicum miliaceum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.20 Panicum miliaceum isoelectric focusing
-
 8.3