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Literature summary extracted from

  • Pitula, J.S.; Deck, K.M.; Clarke, S.L.; Anderson, S.A.; Vasanthakumar, A.; Eisenstein, R.S.
    Selective inhibition of the citrate-to-isocitrate reaction of cytosolic aconitase by phosphomimetic mutation of serine-711 (2004), Proc. Natl. Acad. Sci. USA, 101, 10907-10912.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.3 S711A citrate-to-isocitrate aconitase activity is about 70% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 90% of wild-type activity Homo sapiens
4.2.1.3 S711D no citrate-to-isocitrate aconitase activity, isocitrate-to-cis-aconitate activity is identical to wild-type activity Homo sapiens
4.2.1.3 S711E citrate-to-isocitrate aconitase activity is about 10% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 20% of wild-type activity Homo sapiens
4.2.1.3 S711T citrate-to-isocitrate aconitase activity is about 60% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 60% of wild-type activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.3 cytosol
-
 Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.3 phosphoprotein S711 of IRP1 is phosphorylated by proteinkinase C in vitro and in vivo in human HEK cells. Aconitase activity of the S711 phosphomimetic mutants is preferentially inhibited in the citrate mode of aconitase function. The substitution of phosphomimetic amino acids for S711 fails to affect the RNA-binding affinity of IRP1 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.3 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.3 citrate
-
 Homo sapiens isocitrate
-
 r
4.2.1.3 isocitrate
-
 Homo sapiens cis-aconitate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.3 c-acon
-
 Homo sapiens
4.2.1.3 iron-regulatory protein 1
-
 Homo sapiens
4.2.1.3 IRP1
-
 Homo sapiens