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Literature summary extracted from

  • Naested, H.; Kramhoft, B.; Lok, F.; Bojsen, K.; Yu, S.; Svensson, B.
    Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification (2006), Protein Expr. Purif., 46, 56-63.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 gene agl97, expression of functional His-tagged full-length enzyme in Pichia pastoris, production by high cell-density fermentation Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.7
-
 maltose pH 4.5, 37°C, recombinant enzyme Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 92000
-
 wild-type enzyme, gel filtration Hordeum vulgare
3.2.1.20 100000
-
 recombinant enzyme, gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 maltose + H2O Hordeum vulgare
-
 alpha-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Hordeum vulgare Q9LLY2 gene agl97
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant enzyme 25fold from Pichia pastoris by dia- and ultrafiltration, nickel affinity chromatography and gel filtration Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 25.6
-
 purified recombinant enzyme Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 maltose + H2O
-
 Hordeum vulgare alpha-D-glucose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 100000, recombinant enzyme, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 high pI alpha-glucosidase
-
 Hordeum vulgare
3.2.1.20 More the enzyme belongs to the alpha-glucosidase family 31 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 37
-
 assay at Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 81.7
-
 maltose pH 4.5, 37°C, recombinant enzyme Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5
-
 assay at Hordeum vulgare