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Literature summary extracted from
- Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing (2006), RNA, 12, 1104-1115.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.26.11 | - |
Drosophila melanogaster |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.11 | C442A | 12fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | C467A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | D466A | 1500fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | E469A | 300fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | G438A | 5000fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | G440A | 4fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | G468A | 900fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | G470A | 13fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | G473A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | G480A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | I443A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | I475A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | K446A | 25fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | L437A | 7fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | L464A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | L465A | 10fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | L478A | 11fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | N445A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | N449A | 80fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | P444A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | Q474A | 750fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | R448A | 625fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | R477A | 2600fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | S441A | 9fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | T439A | 2.5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | T447A | 2fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | T471A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | V450A | 4fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | V463A | 6fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | V476A | 6fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | Y472A | 4fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| 3.1.26.11 | Y479A | 7fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.11 | 0.0000198 | - |
pre-tRNAHis | - |
Drosophila melanogaster | |
| 3.1.26.11 | 0.0000394 | - |
pre-tRNAHis-AUG | - |
Drosophila melanogaster | |
| 3.1.26.11 | 0.0000401 | - |
pre-tRNAHis-CCA | - |
Drosophila melanogaster |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.11 | Drosophila melanogaster | Q8MKW7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.11 | - |
Drosophila melanogaster |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.26.11 | cell culture | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.11 | pre-tRNAHis + H2O | - |
Drosophila melanogaster | ? | - |
? |  |
| 3.1.26.11 | pre-tRNAHis-AUG + H2O | - |
Drosophila melanogaster | ? | - |
? | |
| 3.1.26.11 | pre-tRNAHis-CCA + H2O | - |
Drosophila melanogaster | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.11 | tRNase Z | - |
Drosophila melanogaster |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.11 | 0.0049 | - |
pre-tRNAHis-CCA | - |
Drosophila melanogaster | |
| 3.1.26.11 | 0.083 | - |
pre-tRNAHis | - |
Drosophila melanogaster | |
| 3.1.26.11 | 0.365 | - |
pre-tRNAHis-AUG | - |
Drosophila melanogaster |
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