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Literature summary extracted from

  • Jaindl, M.; Popp, M.
    Cyclitols protect glutamine synthetase and malate dehydrogenase against heat induced deactivation and thermal denaturation (2006), Biochem. Biophys. Res. Commun., 345, 761-765.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.2 glycinebetaine 0.4 M, 67.7% residual activity after 60°C heat treatment Escherichia coli
6.3.1.2 additional information glucose shows little tendency to protect the enzyme from loss of activity Escherichia coli
6.3.1.2 additional information glucose shows little tendency to protect the enzyme from loss of activity Hordeum vulgare
6.3.1.2 myo-inositol 0.4 M protects enzymatic activity during heat treatment Hordeum vulgare
6.3.1.2 myo-inositol 0.4 M, 70.1% residual activity after 60°C heat treatment Escherichia coli
6.3.1.2 pinitol 0.4 M results in maintenance of activity to 90% and 50% during heat treatment Hordeum vulgare
6.3.1.2 pinitol 0.4 M, 74.2% residual activity after 60°C heat treatment Escherichia coli
6.3.1.2 proline 0.4 M, 72.2% residual activity after 60°C heat treatment Escherichia coli
6.3.1.2 quercitol 0.4 M, 75.5% residual activity after 60°C heat treatment Escherichia coli
6.3.1.2 sorbitol 0.4 M, 62.0% residual activity after 60°C heat treatment Escherichia coli

General Stability

EC Number General Stability Organism
6.3.1.2 myo-inositol (0.4 M) protects enzymatic activity during heat treatment, pinitol (0.4 M) results in maintenance of activity to 90% and 50% during heat treatment, glucose shows little tendency to protect the enzyme from loss of activity Hordeum vulgare
6.3.1.2 with myo-inositol (0.4 M) 70.1% residual activity after 60°C heat treatment, with pinitol (0.4 M) 74.2% residual activity after 60°C heat treatment, with quercitol (0.4 M) 75.5% residual activity after 60°C heat treatment, with sorbitol (0.4 M) 62.0% residual activity after 60°C heat treatment, with proline (0.4 M) 72.2% residual activity after 60°C heat treatment, with glycinebetain (0.4 M) 67.7% residual activity after 60°C heat treatment, glucose shows little tendency to protect the enzyme from loss of activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Sus scrofa
-
-
-
6.3.1.2 Escherichia coli
-
-
-
6.3.1.2 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 Makroprep HighQ chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 heart
-
 Sus scrofa
-
6.3.1.2 leaf
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Escherichia coli ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Hordeum vulgare ADP + phosphate + L-Gln
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 L-Glutamine synthetase
-
 Escherichia coli
6.3.1.2 L-Glutamine synthetase
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 additional information
-
 0.4 M myo-inositol raises the Tm-value of the enzyme by 3.4°C, pinitol increases Tm by 3.8°C, glucose has no effect Sus scrofa
6.3.1.2 35
-
 retains normal activity after heating for 5 min at 35°C Hordeum vulgare
6.3.1.2 40 55 activity decreases to 50% on heating at 40°C and to 10% or less on incubation at 55°C Hordeum vulgare
6.3.1.2 60 80 deactivation in the temperature range 60-80°C Escherichia coli